REG1B / PSPS2 Protein (His Tag)

Regenerating Islet-Derived 1 beta ( REG1B / PSPS2 ) Protein

REG1B / PSPS2 Protein Product Information

• Synonym: PSPS2, REGH, REGI-BETA, REGL
• Protein Construction: A DNA sequence encoding the human REG1B ( NP_006498.1 ) ( Met 1 - Asn 166 ) was fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

REG1B / PSPS2 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 23
• Molecular Mass: The recombinant human REG1B consists of 155 amino acids and predictes a molecular mass of 17.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh REG1B is approximately 19-21 kDa due to different glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: REG1B protein

REG1B / PSPS2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

REG1B / PSPS2 Protein Description

Regenerating gene (Reg), first isolated from a regenerating islet cDNA library, encodes a secretory protein with a growth stimulating effect on pancreatic beta cells. Reg and Reg-related genes which were expressed in various organs have been revealed to constitute a multigene family, the Reg family consisting of four subtypes (types I, II, III, IV) and are involved in cancers and neurodegenerative diseases. Regenerating islet-derived 1 beta (REG1B), also known as Lithostathine-1-beta and Pancreatic stone protein 2 (PSPS2), is a types I Reg protein and contains one typical C-type lectin domain. REG1B is a 166-amino acid protein which has 22 amino acid substitutions in comparison with the previously isolated human REG1A. REG1B Is normally found in the exocrine pancreas, whereas in other tissues it appears either only under pathological conditions, such as Alzheimer's disease (brain), cancer (colon), or during regeneration such as neuronal sprouting in brain and pancreas regeneration. REG1B might act as an inhibitor of spontaneous calcium carbonate precipitation.

References

1. Moriizumi, S.S. et al., 1994, Biochim. Biophys. Acta. 1217: 199-202.
2. De Reggi, M et al., 1995, Eur, J. Biochem. 230: 503-10.
3. Bartoli, C. et al., 1993, FEBS. Lett. 327: 289-93.