REG1B / PSPS2 Antibody (Antigen Affinity Purified)

REG1B / PSPS2 Antibody ( Antigen Affinity Purified )

REG1B / PSPS2 Antibody Product Information

• Immunogen: Recombinant human REG1B protein ( Catalog#11638-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human REG1B ( rh REG1B ; Catalog#11638-H08H ; NP_006498.1 ; Met 1 - Asn 166 ). REG1B specific IgG was purified by human REG1B affinity chromatography

REG1B / PSPS2 Antibody Usage Guide

• Specificity: Human REG1B / PSPS2
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human REG1B in WB. Using a DAB detection system, the detection limit for human REG1B is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human REG1B. The detection limit for human REG1B is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

REG1B / PSPS2 Antibody Background

Regenerating gene (Reg), first isolated from a regenerating islet cDNA library, encodes a secretory protein with a growth stimulating effect on pancreatic beta cells. Reg and Reg-related genes which were expressed in various organs have been revealed to constitute a multigene family, the Reg family consisting of four subtypes (types I, II, III, IV) and are involved in cancers and neurodegenerative diseases. Regenerating islet-derived 1 beta (REG1B), also known as Lithostathine-1-beta and Pancreatic stone protein 2 (PSPS2), is a types I Reg protein and contains one typical C-type lectin domain. REG1B is a 166-amino acid protein which has 22 amino acid substitutions in comparison with the previously isolated human REG1A. REG1B Is normally found in the exocrine pancreas, whereas in other tissues it appears either only under pathological conditions, such as Alzheimer's disease (brain), cancer (colon), or during regeneration such as neuronal sprouting in brain and pancreas regeneration. REG1B might act as an inhibitor of spontaneous calcium carbonate precipitation.

References

1. Moriizumi, S.S. et al., 1994, Biochim. Biophys. Acta. 1217: 199-202.
2. De Reggi, M et al., 1995, Eur, J. Biochem. 230: 503-10.
3. Bartoli, C. et al., 1993, FEBS. Lett. 327: 289-93.