REG1A / PSPS Protein (His Tag)

Regenerating Islet-Derived Protein 1-alpha Protein

REG1A / PSPS Protein Product Information

• Synonym: REG1A, ICRF, MGC12447, P19, PSP, PSPS, PSPS1, PTP, R EG
• Protein Construction: A DNA sequence encoding the human REG1A ( NP_002900.2 ) ( Met 1- Asn 166 ) precursor was fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

REG1A / PSPS Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 23
• Molecular Mass: The recombinant human REG1A consists of 155 amino acids after removal of the signal peptide and has a predicted molecular mass of 17.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh REG1A is approximately 19 kDa
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: REG1A protein

REG1A / PSPS Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

REG1A / PSPS Protein Description

Lithostathine-1-alpha, also known as Pancreatic stone protein, Pancreatic thread protein, Regenerating islet-derived protein 1-alpha, REG1A, REG-1-alpha, and PSPS, is highly expressed in fetal and infant brains. REG1A contains one C-type lectin domain and is a known growth factor affecting pancreatic islet beta cells. REG1A may act as an inhibitor of spontaneous calcium carbonate precipitation. It may also be associated with neuronal sprouting in brain, and with brain and pancreas regeneration. REG1A has been reported to be expressed in human cancers, and it may be positively correlated with patient's prognosis. REG3A and REG1A proteins are both involved in liver and pancreatic regeneration and proliferation. High levels of REG1A expression by tumor cells are an independent predictor of a poor prognosis in patients with non-small cell lung cancer (NSCLC).

References

1. Gerbaud, V. et al.,2000, J. Biol. Chem. 275: 1057-1064.
2. Cavard, C. et al., 2006, Oncogene. 25 (4): 599-608.
3. Astrosini, C. et al., 2008, Int J Cancer. 123 (2): 409-13.
4. Minamiya, Y. et al., 2008, Lung Cancer. 60 (1): 98-104.
5. Geng, J. et al., 2009, Eur J Surg Oncol. 35 (8): 852-7.