Rat REG1A / PSPS Protein

Regenerating islet-derived 1 alpha Protein Datasheet

REG1A / PSPS Protein Product Information

• Synonym: REG1A, Reg1,Reg
• Protein Construction: A DNA sequence encoding the rat REG1A (P10758) (Met1-Asn100) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

REG1A / PSPS Protein QC Testing

• Purity: (55.3+31.4)% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 23
• Molecular Mass: The recombinant rat REG1A /Fc is a disulfide-linked homodimer. The reduced monomer comprises 319 amino acids and has a predicted molecular mass of 35.8 kDa. The apparent molecular mass of the protein is approximately 40 and 43 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: REG1A / PSPS protein

REG1A / PSPS Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

REG1A / PSPS Protein Description

Lithostathine-1-alpha, also known as Pancreatic stone protein, Pancreatic thread protein, Regenerating islet-derived protein 1-alpha, REG1A, REG-1-alpha, and PSPS, is highly expressed in fetal and infant brains. REG1A contains one C-type lectin domain and is a known growth factor affecting pancreatic islet beta cells. REG1A may act as an inhibitor of spontaneous calcium carbonate precipitation. It may also be associated with neuronal sprouting in brain, and with brain and pancreas regeneration. REG1A has been reported to be expressed in human cancers, and it may be positively correlated with patient's prognosis. REG3A and REG1A proteins are both involved in liver and pancreatic regeneration and proliferation. High levels of REG1A expression by tumor cells are an independent predictor of a poor prognosis in patients with non-small cell lung cancer (NSCLC).

References

1. Gerbaud V. et al.,2000, J Biol Chem. 275: 1057-64.
2. Cavard C. et al., 2006, Oncogene. 25 (4): 599-608.
3. Astrosini C. et al., 2008, Int J Cancer. 123 (2): 409-13.
4. Minamiya Y. et al., 2008, Lung Cancer. 60 (1): 98-104.
5. Geng J. et al., 2009 Eur J Surg Oncol. 35 (8): 852-7.