REG1A Antibody

REG1A Antibody Datasheet

REG1A Antibody Product Information

• Immunogen: Recombinant Human REG1A protein (Catalog#11234-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 6F8H10D4
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human REG1A (rh REG1A; Catalog#11234-H08H; NP_002900.2; Met 1-Asn 166). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

REG1A Antibody Usage Guide

• Specificity: Human REG1A
• No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human REG1A. The detection limit for Human REG1A is approximately 0.078 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

REG1A Antibody Background

Lithostathine-1-alpha, also known as Pancreatic stone protein, Pancreatic thread protein, Regenerating islet-derived protein 1-alpha, REG1A, REG-1-alpha, and PSPS, is highly expressed in fetal and infant brains. REG1A contains oneC-type lectin domain and is a known growth factor affecting pancreatic islet beta cells. REG1A may act as an inhibitor of spontaneous calcium carbonate precipitation. It may also be associated with neuronal sprouting in brain, and with brain and pancreas regeneration. REG1A has been reported to be expressed in human cancers, and it may be positively correlated with patient's prognosis. REG3A and REG1A proteins are both involved in liver and pancreatic regeneration and proliferation. High levels of REG1A expression by tumor cells are an independent predictor of a poor prognosis in patients with non-small cell lung cancer (NSCLC).

References

1. Gerbaud, V. et al.,2000, J. Biol. Chem. 275: 1057-1064.
2. Cavard, C. et al., 2006, Oncogene. 25 (4): 599-608.
3. Astrosini, C. et al., 2008, Int J Cancer. 123 (2): 409-13.
4. Minamiya, Y. et al., 2008, Lung Cancer. 60 (1): 98-104.
5. Geng, J. et al., 2009, Eur J Surg Oncol. 35 (8): 852-7.