RCN3 Protein (His Tag)

Reticulocalbin 3, EF-hand calcium binding domain Protein Datasheet

RCN3 Protein Product Information

• Synonym: RCN3
• Protein Construction: A DNA sequence encoding the human RCN3 (Q96D15)(Met1-His324) was expressed with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

RCN3 Protein QC Testing

• Purity: > 90% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Lys 21
• Molecular Mass: The recombinant human RCN3 comprises 315 amino acids and has a predicted molecular mass of 36.2 kDa. The apparent molecular mass of the protein is approximately 47 kDa in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: RCN3 protein

RCN3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

RCN3 Protein Description

RCN3 is a member of the CREC family. CREC family contains multiple EF-hand Ca²⁺-binding proteins localized to the secretory pathway. The elective and transient association of RCN3 with the precursor of PACE4 plays an important role in the biosynthesis of PACE4. RCN3 sequence is characterized by the presence of five Arg-Xaa-Xaa-Arg motifs, which represents the target sequence of subtilisin-like proprotein convertases(SPCs). SPCs are a family of seven structurally related serine endoproteases that are involved in the proteolytic activation of proproteins.RCN3 is transiently associated with proPACE4, but not with mature PACE4. Inhibition of PACE4 maturation by a Ca²⁺ ionophore resulted in accumulation of the proPACE4-RCN-3 complex in cells.

References

1. Danielsen JM. et al., 2011, Mol Cell Proteomics. 10 (3): M110.003590.
2. Rual JF. et al., 2005, Nature. 437 (7062): 1173-8.
3. Kamatani Y. et al., 2010, Nat Genet. 42 (3): 210-5.