RBP4 Antibody

RBP4 Antibody Datasheet

RBP4 Antibody Product Information

• Immunogen: Recombinant Mouse RBP4 protein (Catalog#50170-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 001
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse RBP4 (rM RBP4; Catalog#50170-M08H; NP_035385.1; Met 1-Leu 201).

RBP4 Antibody Usage Guide

• Specificity: Mouse RBP4
• No cross-reactivity in ELISA with
  

  Human RBP4

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse RBP4 in WB. Using a DAB detection system, the detection limit for Mouse RBP4 is approximately 0.5 ng/lane under non-reducing conditions and 0.25 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse RBP4. The detection limit for Mouse RBP4 is approximately 0.0049 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

RBP4 Antibody Background

Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. In addition, RBP4 is recently identified as an adipokine whose dysregulation is closely associated with the pathogenesis of insulin resistance and type 2 diabetes, as well as obesity.

References

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2. Loredana, Q. et al., 2002, J. Biol. Chem. 277:30191-30197.
3. Zanotti, G. et al., 2004, Vitamins and Hormones. 69: 271-295.
4. Cho, Y.M. et al., 2006, Diabetes.Care. 29: 2457-2461.
5. Balagopal, P. et al., 2007, J. Clin. Endocirnol. Metab. 92: 1971-1974.