RBP4 Antibody (Antigen Affinity Purified)

RBP4 Antibody ( Antigen Affinity Purified ) Datasheet

RBP4 Antibody Product Information

• Immunogen: Recombinant Rat RBP4 protein (Catalog#80008-R08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Rat RBP4 (rR RBP4; Catalog#80008-R08H; NP_037294.1; Met 1-Leu 201). RBP4 specific IgG was purified by Rat RBP4 affinity chromatography.

RBP4 Antibody Usage Guide

• Specificity: Rat RBP4
• Immunochemistry: IHC-P: 0.1-2 μg/mL

  

  Immunochemical staining of rat RBP4 in rat kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

  Immunochemical staining of rat RBP4 in rat liver with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat RBP4 in WB. Using a DAB detection system, the detection limit for Rat RBP4 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat RBP4. The detection limit for Rat RBP4 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

RBP4 Antibody Background

Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. In addition, RBP4 is recently identified as an adipokine whose dysregulation is closely associated with the pathogenesis of insulin resistance and type 2 diabetes, as well as obesity.

References

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4. Cho, Y.M. et al., 2006, Diabetes.Care. 29: 2457-2461.
5. Balagopal, P. et al., 2007, J. Clin. Endocirnol. Metab. 92: 1971-1974.