Human RASSF1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13220-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

RASSF1 cDNA Clone Datasheet

RASSF1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_007182.4
cDNA Size:	1023
Gene Synonym:	123F2; RDA32; NORE2A; RASSF1A; REH3P21;RASSF1
Source:	Human

RASSF1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens Ras association (RalGDS/AF-6) domain family member 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

RASSF1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

RASSF1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

RASSF1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCGGGGG AGCCTGAGCT CATTGAGCTG CGGGAGCTGG CACCCGCTGG GCGCGCTGGG
61       AAGGGCCGCA CCCGGCTGGA GCGTGCCAAC GCGCTGCGCA TCGCGCGGGG CACCGCGTGC
121      AACCCCACAC GGCAGCTGGT CCCTGGCCGT GGCCACCGCT TCCAGCCCGC GGGGCCCGCC
181      ACGCACACGT GGTGCGACCT CTGTGGCGAC TTCATCTGGG GCGTCGTGCG CAAAGGCCTG
241      CAGTGCGCGC ATTGCAAGTT CACCTGCCAC TACCGCTGCC GCGCGCTCGT CTGCCTGGAC
301      TGTTGCGGGC CCCGGGACCT GGGCTGGGAA CCCGCGGTGG AGCGGGACAC GAACGTGGAC
361      GAGCCTGTGG AGTGGGAGAC ACCTGACCTT TCTCAAGCTG AGATTGAGCA GAAGATCAAG
421      GAGTACAATG CCCAGATCAA CAGCAACCTC TTCATGAGCT TGAACAAGGA CGGTTCTTAC
481      ACAGGCTTCA TCAAGGTTCA GCTGAAGCTG GTGCGCCCTG TCTCTGTGCC CTCCAGCAAG
541      AAGCCACCCT CCTTGCAGGA TGCCCGGCGG GGCCCAGGAC GGGGCACAAG TGTCAGGCGC
601      CGCACTTCCT TTTACCTGCC CAAGGATGCT GTCAAGCACC TGCATGTGCT GTCACGCACA
661      AGGGCACGTG AAGTCATTGA GGCCCTGCTG CGAAAGTTCT TGGTGGTGGA TGACCCCCGC
721      AAGTTTGCAC TCTTTGAGCG CGCTGAGCGT CACGGCCAAG TGTACTTGCG GAAGCTGTTG
781      GATGATGAGC AGCCCCTGCG GCTGCGGCTC CTGGCAGGGC CCAGTGACAA GGCCCTGAGC
841      TTTGTCCTGA AGGAAAATGA CTCTGGGGAG GTGAACTGGG ACGCCTTCAG CATGCCTGAA
901      CTACATAACT TCCTACGTAT CCTGCAGCGG GAGGAGGAGG AGCACCTCCG CCAGATCCTG
961      CAGAAGTACT CCTATTGCCG CCAGAAGATC CAAGAGGCCC TGCACGCCTG CCCCCTTGGG
1021     TGA

Quality Control:cDNA sequencing results is available upon request.