Human TNFSF11/CD254 Gene cDNA Clone / ORF Clone

TNFSF11 cDNA Clone Datasheet

TNFSF11 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_033012.3
• cDNA Size: 735
• Gene Synonym: ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TRANCE; hRANKL2; TNFSF11
• Source: Human

TNFSF11 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens tumor necrosis factor (ligand) superfamily, member 11 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

TNFSF11 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TNFSF11 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

TNFSF11 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACCCAA ACAGGATTTC TGAGGATGGC ACCCACTGTA TCTACAGGAT TCTGAGACTG
61       CATGAGAATG CTGACTTCCA GGACACCACC TTGGAGAGCC AGGACACCAA ACTGATTCCT
121      GACTCCTGTA GGAGGATTAA GCAGGCTTTC CAGGGAGCAG TCCAGAAGGA ACTCCAACAC
181      ATTGTGGGCA GCCAACACAT CAGGGCTGAG AAGGCTATGG TGGATGGCTC CTGGCTGGAC
241      CTGGCTAAGA GGAGCAAATT GGAGGCTCAA CCATTTGCCC ACCTGACCAT CAATGCCACA
301      GACATCCCAT CTGGCAGCCA CAAGGTGTCC CTGTCCTCCT GGTACCATGA CAGGGGCTGG
361      GGCAAGATTA GCAATATGAC CTTCAGCAAT GGCAAACTGA TTGTGAACCA GGATGGCTTC
421      TACTACCTCT ATGCCAACAT CTGTTTCAGA CACCATGAGA CCTCTGGAGA CCTGGCTACA
481      GAATACCTCC AACTTATGGT CTATGTGACC AAGACCAGCA TCAAGATTCC ATCCAGCCAC
541      ACCCTGATGA AGGGAGGCAG CACCAAATAC TGGTCTGGCA ACTCTGAGTT CCACTTCTAC
601      AGCATCAATG TGGGAGGCTT CTTCAAACTG AGGTCTGGAG AGGAGATTAG CATTGAGGTG
661      AGCAACCCAT CCCTGCTGGA CCCTGACCAG GATGCCACCT ACTTTGGAGC CTTCAAGGTG
721      AGGGACATTG ACTAA

 

Quality Control:cDNA sequencing results is available upon request.