TNFSF11 / RANKL / CD254 Protein

Tumor Necrosis Factor Ligand Superfamily Member 11 Protein

TNFSF11 / RANKL / CD254 Protein Product Information

• Synonym: TNFSF11, RP11-86N24.2, CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf
• Protein Construction: A DNA sequence encoding the human TNFSF11 isoform 2 (O14788-2) (Gly 63- Asp 244) was fused with the Fc region of human IgG1 at the N-terminus
• Source: Human
• Expression Host: Human Cells

TNFSF11 / RANKL / CD254 Protein QC Testing

• Purity: > 86 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 20
• Molecular Mass: The recombinant human TNFSF11/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 443 amino acids and has a calculated molecular mass of 48.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh TNFSF11/Fc monomer is approximately 50-55 kDa due to the glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
• SDS-PAGE: TNFSF11 protein

TNFSF11 / RANKL / CD254 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TNFSF11 / RANKL / CD254 Protein Description

Tumor necrosis factor ligand superfamily member 11, also known as Receptor activator of nuclear factor kappa-B ligand, Osteoprotegerin ligand, TNFSF11, RANKL, TRANCE, OPGL and CD254, is a single-pass type I I membrane protein which belongs to the tumor necrosis factor family. TNFSF11 is a cytokine which binds to TNFRSF11B / OPG and to TNFRSF11A / RANK. It is an osteoclast differentiation and activation factor. TNFSF11 augments the ability of dendritic cells to stimulate naive T-cell proliferation. It may be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. TNFSF11 may also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2), also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone.

References

1. Anderson DM. et al., 1997, Nature 390:175-9.
2. Nagai M. et al., 2000, Biochem. Biophys. Res. Commun. 269:532-6.
3. Sobacchi C. et al., 2007, Nat. Genet. 39: 960-2.