RACK1 / GNB2L1 Protein (MBP Tag)

Guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 Protein Datasheet

RACK1 / GNB2L1 Protein Product Information

• Synonym: HLC7, Gnb2-rs1, H12.3, HLC-7, PIG21, RACK1
• Protein Construction: A DNA sequence encoding the human GNB2L1 (P63244 ) (Met 1-Arg 317) was fused with an N-terminal polyhistidine-tagged MBP tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

RACK1 / GNB2L1 Protein QC Testing

• Purity: > 83 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human GNB2L1/MBP fusion protein consists of 714 amino acids and has a calculated molecular mass of 78.7 KDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.5.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: RACK1 / GNB2L1 protein

RACK1 / GNB2L1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

RACK1 / GNB2L1 Protein Description

RACK1 (guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1), also known as GNB2L1, belongs to the WD repeat G protein beta family. RACK1 facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. It modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. RACK1 is involved in PKC-dependent translocation of ADAM12 to the cell membrane. GNB2L1 promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. It is required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. GNB2L1 also is required for PTK2 phosphorylation and dephosphorylation. It regulates internalization of the muscarinic receptor CHRM2. It also promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L.

References

1. Jannot G. et al., 2011, EMBO Rep. 12 (6): 581-6.
2. Wang F. et al., 2011, J Biol Chem. 286 (11): 9097-106.
3. Cao XX. et al., 2011, Breast Cancer Res Treat. 126 (3): 555-63.
4. Myklebust LM. et al., 2011, Thyroid. 21 (11): 1217-25.