- EGFR Signaling Pathway
- TGF-beta Signaling
- Canonical Wnt Signaling
- non-Canonical Wnt Signaling
- Notch Signaling
- p53 Pathway
- NF-kB Pathway
- Cytokine Signaling
>CHO Cell Expressed
>Mouse R-Spondin 1 / RSPO1 Protein (His Tag)
|Catalog||Size (Price)||Quantity||In Stock||Operation|
|5µg promotion size is available before 31 Dec 2013|
R-spondin homolog (Xenopus laevis) Protein Datasheet
R-Spondin 1 / RSPO1 Protein Price Inquiry ( Available Sizes )
R-Spondin 1 / RSPO1 Protein Product Information
|Synonym :||RP23-325M14.2, R-spondin, Rspondin|
A DNA sequence encoding the full length of mouse RSPO1 (NP_619624.2) (Met 1-Gln 265) was fused with a polyhistidine tag at the C-terminus.
|Expression Host:||CHO Stable Cell|
R-Spondin 1 / RSPO1 Protein QC Testing
|Purity:||> 95 % as determined by SDS-PAGE||SDS-PAGE:
R-Spondin 1 / RSPO1 protein
|Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.
The ED50 for this effect is typically 50-200 ng/ml in the presence of 50 ng/ml recombinant mouse Wnt3a.
|Endotoxin:||< 1.0 EU per μg of the protein as determined by the LAL method|
|Stability:||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N terminal:||Ser 21|
The secreted recombinant mouse RSPO1 comprises 256 amino acids with a predicted molecular mass of 28.5 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 44 kDa in SDS-PAGE under reducing conditions.
|Formulation:||Lyophilized from sterile PBS, pH7.4.
R-Spondin 1 / RSPO1 Protein Usage Guide
|Storage:||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution:||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
R-Spondin 1 / RSPO1 Protein Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|RSPO1||Human||RSPO1 Protein, Recombinant||11083-H08H|
|RSPO1||Human||RSPO1 (aa 1-146) Protein, Recombinant||11083-H08H1|
|RSPO1||Mouse||RSPO1 Protein, Recombinant||50316-M08H|
|RSPO1||Mouse||R-Spondin 1 / RSPO1 Protein, Recombinant||50316-M08S|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|WB||RSPO1 Antibody, Rabbit MAb||11083-R010|
|ELISA||RSPO1 Antibody, Rabbit MAb||11083-R106|
|WB, ELISA||Rabbit Polyclonal Antibody||11083-RP01|
|WB, ELISA||Rabbit Polyclonal Antibody (Antigen Affinity Purified)||11083-RP02|
R-Spondin 1 / RSPO1 Protein Description
R-Spondin 1 ( Roof plate-specific Spondin 1 ), also known as RSPO1 and Cristin 3, is a secreted protein which belongs to the R-Spondin family. The R-Spondin (RSPO) family of secreted proteins act as potent activators of the Wnt/beta-catenin signaling pathway. R-Spondin1 regulates cellular responsiveness to Wnt ligands by modulating the cell-surface levels of the coreceptor LRP6. R-Spondin1 activity critically depends on the presence of canonical Wnt ligands and LRP6. R-Spondin1 does not directly activate LRP6, it interferes with DKK1/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 levels on the cell surface. Mature human R-Spondin1 is 27 kDa and 243 amino acids (aa) in length. It contains a cysteine-rich furin repeat domain followed by a type I TSP domain. This form is apparently retained intracellularly. Human R-Spondin1 shares 89%, 87%, 92%, 91%, 91%, and 89% aa identity with mouse, rat, equine, canine, caprine and bovine R-Spondin1, respectively. Human RSPO1 disruption results in a recessive syndrome characterized by XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. It has been shown that the complete female-to-male sex reversal is due to the absence of the testis-determining gene, SRY.
- Parma, P. et al., 2006, Nature genetics. 38 (11): 1304-9.
- Capel, B., 2006, Nature genetics. 38 (11) :1233-4.
- Binnerts, ME. et al., 2007, Proc Natl Acad Sci. 104 (37): 14700-5.
- Wilhelm, D., 2007, Bioessays. 29 (4): 314-8.
- Zhao, J. et al., 2009, Proc Natl Acad Sci. 106 (7) :2331-6.