Anti-Nucleoside phosphorylase Antibody

Cat: 12097-R103
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Anti-Nucleoside phosphorylase Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Nucleoside phosphorylase Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human Nucleoside phosphorylase
Immunogen
Recombinant Human Purine nucleoside phosphorylase / PNP protein (Catalog#12097-H08E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Purine nucleoside phosphorylase / PNP (rh Purine nucleoside phosphorylase / PNP; Catalog#12097-H08E; P00491; Met 1-Ser 289).
Source
Monoclonal Rabbit IgG Clone #103
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Nucleoside phosphorylase Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Nucleoside phosphorylase Antibody (Rabbit Monoclonal antibody) Images
Human PNP Western blot (WB) 13469

Anti-PNP3 rabbit monoclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: K562 Whole Cell Lysate

Lane C: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:32 kDa

Observed band size:32 kDa

Human PNP Immunoprecipitation(IP) 17700

PNP-3 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg K562 Whole Cell Lysate

2 µL anti-PNP-3 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-PNP-3 rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 33 kDa

Observed band size: 33 kDa

Anti-Nucleoside phosphorylase Antibody Alternative Names
Anti-NP Antibody;Anti-PRO1837 Antibody;Anti-PUNP Antibody
Nucleoside phosphorylase Background Information
Full Name
purine nucleoside phosphorylase
References
  • de Azevedo,W.F. et al., 2003, Biochem Biophys Res Commun. 312 (3): 767-72.
  • Canduri,F. et al., 2005, Acta Crystallogr D Biol Crystallogr. 61 (Pt 7): 856-62.
  • Perera,G.K. et al., 2005,Clin Exp Dermatol. 30 (1):27-9.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Nucleoside phosphorylase Antibody Rabbit Monoclonal IHC-P 12097-R001
    Anti-Nucleoside phosphorylase Antibody Rabbit Polyclonal ELISA 12097-RP01
    Anti-Nucleoside phosphorylase Antibody Rabbit Polyclonal WB,ELISA,IP 12097-T52
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