Purine nucleoside phosphorylase / PNP Antibody

Purine nucleoside phosphorylase / PNP Antibody Datasheet

Purine nucleoside phosphorylase / PNP Antibody Product Information

• Immunogen: Recombinant Human Purine nucleoside phosphorylase / PNP protein (Catalog#12097-H08E)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 103
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human Purine nucleoside phosphorylase / PNP (rh Purine nucleoside phosphorylase / PNP; Catalog#12097-H08E; P00491; Met 1-Ser 289).

Purine nucleoside phosphorylase / PNP Antibody Usage Guide

• Specificity: Human Purine nucleoside phosphorylase / PNP
• Western blot: This antibody can be used at 2-5 μg/mL with the appropriate secondary reagents to detect Human PNP in WB.

  A B C Sample (whole cell lysate) Jurkat K562 293T Sample Volume (cells/lane) 2.5×105 5×105 5×105 Gel 13% SDS-PAGE reducing gel Recommended Concentration 2-5 μg/ml Secondary Antibody Dylight 800-Labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution.

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human PNP. The detection limit for Human PNP is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Purine nucleoside phosphorylase / PNP Antibody Background

Purine nucleoside phosphorylase, also known as Inosine phosphorylase and PNP, is a cytoplasm protein which belongs to thePNP / MTAP phosphorylase family. Purine nucleoside phosphorylase / PNP is a key enzyme in the purine-salvage pathway, which allows cells to utilize preformed bases and nucleosides in order to synthesize nucleotides. PNP is specific for purine nucleosides in the beta-configuration and exhibits a strong preference for purines containing a 6-keto group and ribosyl-containing nucleosides relative to the corresponding analogues. Purine nucleoside phosphorylase (PNP) catalyzes the phosphorolysis of the N-ribosidic bonds of purine nucleosides and deoxynucleosides. PNP is a target for inhibitor development aiming at T-cell immune response modulation and has been submitted to extensive structure-based drug design. Defects in PNP are the cause of purine nucleoside phosphorylase deficiency (PNP deficiency) which leads to a severe T-cell immuno-deficiency with neurologic disorder in children.

References

1. de Azevedo,W.F.et al., 2003, Biochem Biophys Res Commun. 312 (3): 767-72.
2. Canduri,F. et al., 2005, Acta Crystallogr D Biol Crystallogr. 61 (7): 856-62.
3. Perera,G.K. et al., 2005, Clin Exp Dermatol. 30 (1): 27-9.