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Protein L Agarose Beads / Resin

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    Introduction
     

    Sino Biological rProtein L resin was produced by immobilizing recombinant protein L to 4% cross-linked agarose matrix. It is designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins.

     
    Specifications
     
    Ligand Recombinant protein L
    Binding capacity 10-15 mg human IgG/ml
    Matrix Spherical, 4%  agarose
    Average particle size 90μm(45-165μm)
    Maximum linear flow >150 cm/h
    Maximum Pressure 0.3 MPa
    pH stability pH 3-9
    Storage 20% ethanol at 2°C to 8°C
     
    Experiment
     
    Column: 5x 50 mm, bed volume 1.0 ml
    Resin: Protein L agarose resin
    Binding Buffer: 50 mM Tris, 100 mM NaCl, pH 8.0
    Elution Buffer: 100 mM glycin, 10 mM NaCl, pH 3.0
    Sample: humanized monoclonal antibody,1.9 mg/ml
    Flow Rate: 0.3 ml/min
    Procedure: The column was equilibrated with binding buffer, then apply the sample at fow rate of 0.3 ml/min, when the flowthrough concentration reach 0.19 mg/min stop loading and wash the column with binding buffer, when asorbency of 280 nm decrease to base line, elute with elution buffer.
    Result: 16 mg antibody was recovered in eluant, Dynamic binding capacity of the column is about 16 mg/ml.

     

     

    Chromatography graph of measuring dynamic binding capacity

     
    Protein L Resin: Repeated Cycle Usage
     

    The resin is reusable with proper regeneration procedure, no significant recovery loss or performance change after 20 cycle usage.

    SDS-PAGE of monoclonal antibody purified after 20 repeat usage cycle

     
    Protein L Resin: CIP Protocol
     

    Wash the column with 8 M urea, 0.05 M citric acid pH 2.5. Immediately re-equilibrate with at least 5 bed volumes of binding buffer.

     
    Protein L Resin: Column Regeneration
     

    Regenerate column by washing with 3-5 bed volumes of Elution Buffer and then re-equilibration with Binding Buffer. In some applications, substances like denatured proteins or lipids, do not elute in the regeneration procedure. These can be removed by cleaning-in-place procedures.

     
    Protein L Resin: Storage
     

    For storage, keep the medium at 2°C to 8°C in a suitable bacteriostat, e.g. 20% ethanol and/or 0.02% sodium azide. Notice: The resin must not be frozen.

     
    IgG binding of protein A, G, and L
     
    Species

    Protein A

    Protein G

    Protein L

     Human

     IgG

    +++

    +++

    +++

     

     IgG1

    ++++

    ++++

    ++++

     

     IgG2

    ++++

    ++++

    ++++

     

     IgG3

    -

    +++

    +++

     

     IgG4

    ++++

    ++++

    ++++

     

     IgA

    +

    -

    +++

     

     IgA1

    +

    -

    +++

     

     IgA2

    +

    -

    +++

     

     IgD

    -

    -

    +++

     

     IgE

    ++

    -

    +++

     

     IgM

    +

    -

    +++

     Rabbit

     IgG

    +++

    +++

    +

     Cow

     IgG

    +

    +++

    -

     

     IgG1

    +

    +++

    -

     

     IgG2

    +++

    +++

    -

     Cat

     IgG

    +++

    +

    ?

     Horse    

     IgG

    ++

    ++++

    ?

     Goat

     IgG

    +

    ++

    -

     

     IgG1

    +

    +++

    -

     

     IgG2

    +++

    +++

    -

     Guinea-pig

     IgG1

    ++

    +

    ?

     

     IgG2

    ++

    +

    ?

     Sheep

     IgG

    +

    ++

    -

     

     IgG1

    +

    ++

    -

     

     IgG2

    +++

    +++

    -

     Dog

     

    ++

    +

    ?

     Pig

     

    +++

    ++

    +++

     Rat

     IgG

    +

    ++

    +++

     

     IgG1

    -

    +

    +++

     

     IgG2a

    -

    ++++

    +++

     

     IgG2b

    -

    ++

    +++

     

     IgG2c

    ++

    ++

    +++

     

     IgG3

    +

    ++

    ?

     Mouse

     IgG

    ++

    ++

    +++

     

     IgG1

    +

    ++++

    +++

     

     IgG2a

    ++++

    ++++

    +++

     

     IgG2b

    +++

    +++

    +++

     

     IgG3

    ++

    +++

    +++

     

     IgM

    -

    -

    +++

     Chicken

     IgY

    -

    -

    -

     Monkey(rhesus)

     IgG

    ++++

    ++++

    ?

     Hamster

     

    +

    ++

    +++

     Koala

     

    -

    +

    ?

     Llama

     

    -

    +

    ?

    Strong binding ++, medium interaction +, weak or no interaction -.

     
    Related products
     
    Ultra-linked rProtein A resin
    rProtein G resin
    Recombinant Protein A
    Recombinant Protein G
    Recombinant Protein L

     

    Size / Price
    Catalog: 11044-H07E-RN-25
    List Price: 
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    Datasheet & Documentation

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