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Protein G resin PDF Download

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13103-PNAE-RN

  YES          

Introduction

Sino Biological rProtein G resin was produced by immobilizing recombinant protein G to 4% cross-linked agarose matrix. It is designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins or recombinant proteins containing Fc regions from biological fluids or cell culture media.

 

Specifications

Ligand Recombinant Streptococcal Protein G
Dynamic binding capacity ~18 mg/ml
Matrix Spherical, 4% agarose
Average particle size 90um(45-165um)
Maximum linear flow rate >150 cm/h
Maximum Pressure: 0.3 MPa
pH stability  Long term:
Short term:
pH 3-9
pH 2-10
Storage 20% ethanol at 2°C to 8°C

 

IgG binding of protein A, G, and L

 

Species

Protein A

Protein G

Protein L

 Human

 IgG

+++

+++

+++

 

 IgG1

++++

++++

++++

 

 IgG2

++++

++++

++++

 

 IgG3

-

+++

+++

 

 IgG4

++++

++++

++++

 

 IgA

+

-

+++

 

 IgA1

+

-

+++

 

 IgA2

+

-

+++

 

 IgD

-

-

+++

 

 IgE

++

-

+++

 

 IgM

+

-

+++

 Rabbit

 IgG

+++

+++

+

 Cow

 IgG

+

+++

-

 

 IgG1

+

+++

-

 

 IgG2

+++

+++

-

 Cat

 IgG

+++

+

?

 Horse    

 IgG

++

++++

?

 Goat

 IgG

+

++

-

 

 IgG1

+

+++

-

 

 IgG2

+++

+++

-

 Guinea-pig

 IgG1

++

+

?

 

 IgG2

++

+

?

 Sheep

 IgG

+

++

-

 

 IgG1

+

++

-

 

 IgG2

+++

+++

-

 Dog

 

++

+

?

 Pig

 

+++

++

+++

 Rat

 IgG

+

++

+++

 

 IgG1

-

+

+++

 

 IgG2a

-

++++

+++

 

 IgG2b

-

++

+++

 

 IgG2c

++

++

+++

 

 IgG3

+

++

?

 Mouse

 IgG

++

++

+++

 

 IgG1

+

++++

+++

 

 IgG2a

++++

++++

+++

 

 IgG2b

+++

+++

+++

 

 IgG3

++

+++

+++

 

 IgM

-

-

+++

 Chicken

 IgY

-

-

-

 Monkey(rhesus)

 IgG

++++

++++

?

 Hamster

 

+

++

+++

 Koala

 

-

+

?

 Llama

 

-

+

?

 

Strong binding ++, medium interaction +, weak or no interaction -.

Related products

 

Ultra-linked rProtein A resin
rProtein L resin
Recombinant Protein A
Recombinant Protein G
Recombinant Protein L