|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Homo sapiens protein C (inactivator of coagulation factors Va and VIIIa) DNA.|
|pMD18-T Simple Vector|
|Identical with the Gene Bank Ref. ID sequence except for two point mutations: 423 G/T and 459 G/A, neither of which results in any amino acid variation.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human Protein C cDNA ORF Clone, expression ready, FLAG-tagged||HG10196-M-F|
|Human Protein C Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG10196-M-H|
|Human Protein C Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG10196-M-M|
|Human Protein C Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG10196-M-N|
|Human Protein C Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG10196-M-Y|
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