Protein C Inhibitor / SerpinA5 Antibody

SerpinA5 Antibody Datasheet

SerpinA5 Antibody Product Information

• Immunogen: Recombinant Human SerpinA5 protein (Catalog#10309-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 110
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human Protein C Inhibitor / SerpinA5 (rh Protein C Inhibitor / SerpinA5; Catalog#10309-H08H; NP_000615.3; Met 1-Pro 406).

SerpinA5 Antibody Usage Guide

• Specificity: Human SerpinA5
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SerpinA5. The detection limit for Human SerpinA5 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SerpinA5 Antibody Background

Serpin A5, also well known as protein C inhibitor (PCI), is a member of the human serpin superfamily consists of at least 35 members. It is synthesized in the liver and has been detected in saliva, cerebral spinal fluid, amniotic fluid, tears and semen. As a potent inhibitor of the protein C anticoagulant pathway at the levels of both zymogen activation and enzyme inhibition, serpinA5 additionally inhibits a variety of serine protease including thrombin, factor Xa, several kallekreins and acrosin, and plays a role in the processes of blood coagulation and fertilization. Serpin A5 also inhibits urinary plasminogen activator (uPA), a mediator of tumor cell invasion, and regulates tumor growth and metastasis by inhibiting angiogenesis. Furthermore, recent studies have identified PCI as a potent and direct inhibitor of activated HGFA (hepatocyte growth factor activator), suggesting a novel function in the regulation of tissue repair and regeneration. Similar to serpins C1 and D1, the thrombin inhibitory activity of serpinA5 is enhanced by heparin.

References

1. Alireza, R. et al., 1995, J. Biol. Chem. 270: 25336-25339.
2. Silverman, G.A. et al., 2001, J. Biol. Chem. 276: 33293-33296.
3. Malleier, J.M. et al., 2007, Blood. 109: 4769-4776.
4. Asanuma, K. et al., 2007, Int. J. Cancer. 121: 955-965.
5. Hayashi, T. et al., 2007, J. Thromb. Haemost. 5: 1477-1485.