SerpinA5 / PROCI Protein (His Tag)

Serpin Peptidase Inhibitor Clade A Member 5 / SerpinA5 Protein

SerpinA5 / PROCI Protein Product Information

• SerpinA5 Synonym: PAI3, PCI, PLANH3, PROCI
• Protein Construction: A DNA sequence encoding the human SerpinA5 precursor ( NP_000615.3 ) ( Met 1 - Pro 406 ) was expressed with a C-terminal polyhistidine tag.
• Source: Human
• Expression Host: Human Cells

SerpinA5 / PROCI Protein QC Testing

• Purity: > 97% as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His 20
• Molecular Mass: The recombinant human SerpinA5 consists of 398amino acids and predicts amolecular mass of 45 kDa. By SDS-PAGE, the apparent molecular mass of rhSerpinA5 is approximately 50-55 kDa due to the glycosylation.
• Formulation: Lyophilized from sterile PBS, pH 7.2
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: SerpinA5 protein

SerpinA5 / PROCI Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SerpinA5 / PROCI Protein Description

Serpin A5, also well known as protein C inhibitor (PCI), is a member of the human serpin superfamily consists of at least 35 members. It is synthesized in the liver and has been detected in saliva, cerebral spinal fluid, amniotic fluid, tears and semen. As a potent inhibitor of the protein C anticoagulant pathway at the levels of both zymogen activation and enzyme inhibition, serpinA5 additionally inhibits a variety of serine protease including thrombin, factor Xa, several kallekreins and acrosin, and plays a role in the processes of blood coagulation and fertilization. Serpin A5 also inhibits urinary plasminogen activator (uPA), a mediator of tumor cell invasion, and regulates tumor growth and metastasis by inhibiting angiogenesis. Furthermore, recent studies have identified PCI as a potent and direct inhibitor of activated HGFA (hepatocyte growth factor activator), suggesting a novel function in the regulation of tissue repair and regeneration. Similar to serpins C1 and D1, the thrombin inhibitory activity of serpinA5 is enhanced by heparin.

References

1. Alireza, R. et al., 1995, J. Biol. Chem. 270: 25336-25339.
2. Silverman, G.A. et al., 2001, J. Biol. Chem. 276: 33293-33296.
3. Malleier, J.M. et al., 2007, Blood. 109: 4769-4776.
4. Asanuma, K. et al., 2007, Int. J. Cancer. 121: 955-965.
5. Hayashi, T. et al., 2007, J. Thromb. Haemost. 5: 1477-1485.