SerpinA5 Antibody

Plasma Serine Protease Inhibitor ( SerpinA5 ) Antibody

SerpinA5 / Protein C inhibitor Antibody Product Information

• Immunogen: Recombinant human SerpinA5 protein ( Catalog #10309-H08H )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human SerpinA5 (rhSerpinA5; Catalog#10309-H08H; aa 1-406; NP_000615.3). Total IgG was purified by Protein A affinity chromatography.

SerpinA5 / Protein C inhibitor Antibody Usage Guide

• Specificity: Human SerpinA5 / Protein C inhibitor
• Western blot: This antibody can be used at 1 - 2.0 μg/mL with the appropriate secondary reagents to detect human SerpinA5. Using a DAB detection system, the detection limit for human SerpinA5 is approximately 3ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.5 - 1.0 μg/mL with the appropriate secondary reagents to detect human SerpinA5. The detection limit for human SerpinA5 is 0.31ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for 1 month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SerpinA5 / Protein C inhibitor Antibody Background

SerpinA5, also well known as protein C inhibitor (PCI), is a member of the human serpin superfamily. It is synthesized in the liver and has been detected in saliva, cerebral spinal fluid, amniotic fluid, tears and semen. SerpinA5 acts as a potent inhibitor of the protein C anticoagulant pathway at the levels of both zymogen activation and enzyme inhibition. SerpinA5 inhibits a variety of serine protease including thrombin, factor Xa, several kallekreins and acrosin. It plays a critical role in the processes of blood coagulation and fertilization. SerpinA5 also inhibits urinary plasminogen activator (uPA), a mediator of tumor cell invasion, and regulates tumor growth and metastasis by inhibiting angiogenesis. It is a potent and direct inhibitor of activated HGFA (hepatocyte growth factor activator).

References

1. Silverman, G.A. et al., 2001, J. Biol. Chem. 276: 33293-33296.

2. Malleier, J.M. et al., 2007, Blood. 109: 4769-4776.

3. Asanuma, K. et al., 2007, Int. J. Cancer. 121: 955-965.