Mouse ACPP Gene cDNA Clone / ORF Clone

ACPP cDNA Clone Datasheet

ACPP cDNA Clone Product Information

• Gene Bank Ref.ID: NM_207668.2
• cDNA Size: 1254
• Gene Synonym: Lap; PAP; Ppal; AI324033; A030005E02Rik; Acpp
• Source: Mouse

ACPP cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus acid phosphatase, prostate DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 29 G/C resulting in the amino acid Arg substitution by Pro and 126 G/A,174 A/G not causing the amino acid variation.

ACPP cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ACPP cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ACPP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGAGCCG TTCCTCTGCC CCTGAGCCCG ACAGCAAGCC TCAGCCTTGG CTTCTTGCTC
61       CTGCTTTCTC TCTGCCTGGA CCCAGGCCAA GCCAAGGAGT TGAAGTTTGT GACATTGGTG
121      TTTCGACATG GAGACCGAGG TCCCATCGAG ACCTTTCCTA CCGACCCCAT TACGGAATCC
181      TCGTGGCCAC AAGGATTTGG CCAACTCACC CAGTGGGGCA TGGAACAGCA CTACGAACTT
241      GGAAGTTATA TAAGGAAAAG ATACGGAAGA TTCTTGAACG ACACCTATAA GCATGATCAG
301      ATTTATATCC GGAGCACAGA TGTGGACAGG ACTTTGATGA GTGCTATGAC AAACCTTGCA
361      GCCCTGTTTC CTCCAGAGGG GATCAGCATC TGGAATCCTA GACTGCTCTG GCAGCCCATC
421      CCAGTGCACA CCGTGTCTCT CTCTGAGGAT CGGTTGCTGT ACCTGCCTTT CAGAGACTGC
481      CCTCGTTTTG AAGAACTCAA GAGTGAGACT TTAGAATCTG AGGAATTCTT GAAGAGGCTT
541      CATCCATATA AAAGCTTCCT GGACACCTTG TCGTCGCTGT CGGGATTCGA TGACCAGGAT
601      CTTTTTGGAA TCTGGAGTAA AGTTTATGAC CCTTTATTCT GCGAGAGTGT TCACAATTTC
661      ACCTTGCCCT CCTGGGCCAC CGAGGACGCC ATGATTAAGT TGAAAGAGCT ATCAGAATTA
721      TCTCTGCTAT CACTTTATGG AATTCACAAG CAGAAAGAGA AATCTCGACT CCAAGGGGGC
781      GTCCTGGTCA ATGAAATCCT CAAGAATATG AAGCTTGCAA CTCAGCCACA GAAGTATAAA
841      AAGCTGGTCA TGTATTCCGC ACACGACACT ACCGTGAGTG GCCTGCAGAT GGCGCTAGAT
901      GTTTATAATG GAGTTCTGCC TCCCTACGCT TCTTGCCACA TGATGGAATT GTACCATGAT
961      AAGGGGGGGC ACTTTGTGGA GATGTACTAT CGGAATGAGA CCCAGAACGA GCCCTACCCA
1021     CTCACGCTGC CAGGCTGCAC CCACAGCTGC CCTCTGGAGA AGTTTGCGGA GCTACTGGAC
1081     CCGGTGATCT CCCAGGACTG GGCCACGGAG TGTATGGCCA CAAGCAGCCA CCAAGTGCTG
1141     AGGGTTATCC TTGCCACTAC ATTTTGCCTG GTAACCGGGA TCCTGGTGAT ACTTCTGCTT
1201     GTCCTCATCC GCCATGGGCC CTGCTGGCAG AGAGATGTGT ATCGGAACAT CTGA

 

Quality Control:cDNA sequencing results is available upon request.