Prostatic Acid Phosphatase / ACPP Antibody

Prostatic acid Phosphatase / ACPP Antibody Datasheet

Prostatic acid Phosphatase / ACPP Antibody Product Information

• Immunogen: Recombinant Human Prostatic acid Phosphatase / ACPP protein (Catalog#10959-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 03
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Prostatic acid Phosphatase / ACPP (rh Prostatic acid Phosphatase / ACPP; Catalog#10959-H08H; NP_001127666.1; Met 1-Lys 382). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Prostatic acid Phosphatase / ACPP Antibody Usage Guide

• Specificity: Human Prostatic acid Phosphatase / ACPP
• No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human ACPP.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Prostatic acid Phosphatase / ACPP Antibody Background

Prostatic acid phosphatase, also known as ACPP, is a secreted and a single-pass type I membrane protein which belongs to thehistidine acid phosphatase family. ACPP / PSAP is an enzyme produced by the prostate. It may be found in increased amounts in men who have prostate cancer or other diseases. The highest levels of ACPP / PSAP are found in metastasized prostate cancer. Diseases of the bone, such as Paget's disease or hyperparathyroidism, diseases of blood cells, such as sickle-cell disease or multiple myeloma or lysosomal storage diseases, such as Gaucher's disease, will show moderately increased levels. ACPP / PSAP was used to monitor and assess progression of prostate cancer until the introduction of prostate specific antigen (PSA), which has now largely displaced it. ACPP / PSAP has a role in prognosticating intermediate and high-risk prostate cancer, it has led to renewed interest in this marker. Its physiological function may be associated with the liquefaction process of semen. ACPP / PSAP may play an important role in the transmission of HIV. ACPP / PSAP forms fibers made of amyloid. There fibers capture HIV virions promoting their attachment to target cells. The association of ACPP / PSAP with HIV may increase the ability of the virus to infect human cells "by several orders of magnitude."ACPP / PSAP may be a future target of efforts to combat the spread of HIV infection.

References

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4. Zylka MJ, et al.,2008, Neuron. 60 (1): 111-22.