Anti-Prolactin Receptor Antibody

Cat: 10278-R204
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Anti-Prolactin Receptor Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Prolactin Receptor Antibody
Validated applications
ELISA,ELISA(Det),FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human Prolactin Receptor
Immunogen
Recombinant Human PRLR protein (Catalog#10278-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human PRLR (rh PRLR; Catalog#10278-H08H; NP_000940.1; Met 1-Asp 234).
Source
Monoclonal Rabbit IgG Clone #204
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Prolactin Receptor Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  
ICC-IF 1:20-1:100  
FCM 1:25-1:100  
ELISA(Det) 1:1000-1:10000 This antibody will detect Human Prolactin Receptor in ELISA pair set (Catalog: # SEK10278). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10278-R313).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Prolactin Receptor Antibody (Rabbit Monoclonal antibody) Images
Human Prolactin Receptor Immunofluorescence(IF) 2869
Immunofluorescence staining of Human PRLR in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PRLR monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.
Human Prolactin Receptor Flow Cytometry (FC) 5061
Flow cytometric analysis of human PRLR expression on MCF-7 cells. Cells were stained with purified anti-Human PRLR, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Anti-Prolactin Receptor Antibody Alternative Names
Anti-HPRL Antibody;Anti-hPRLrI Antibody;Anti-MFAB Antibody;Anti-PRLR Antibody
Prolactin Receptor Background Information

Prolactin receptor (PRLR) is a single-pass transmembrane receptor belonging to the type â…  cytokine receptor superfamily, and contains two fibronectin type-â…¢ domains. All class 1 ligands activate their respective receptors by clustering mechanisms. Ligand binding results in the transmembrane PRLR dimerization, followed by phosphorylation and activation of the molecules invloved in the signaling pathways, such as Jak-STAT, Ras/Raf/MAPK. The PRLR contains no intrinsic tyrosine kinase cytoplasmic domain but associates with a cytoplasmic tyrosine kinase, JAK2. PRLR mainly serves as the receptor for the pituitary hormone prolactin (PRL), a secreted hormone that affects reproduction and homeostasis in vertebrates. PRLR can be regulated by an interplay of two different mechanisms, PRL or ovarian steroid hormones independently or in combination in a tissue-specific manner. The role of the hormone prolactin (PRL) in the pathogenesis of breast cancer is mediated by its cognate receptor (PRLR). Ubiquitin-dependent degradation of the PRLR that negatively regulates PRL signaling is triggered by PRL-mediated phosphorylation of PRLR on Ser349 followed by the recruitment of the beta-transducin repeats-containing protein (beta-TrCP) ubiquitin-protein isopeptide ligase. which altered PRLR stability may directly influence the pathogenesis of breast cancer.

Full Name
prolactin receptor
References
  • Bole-Feysot C, et al. (1998) Prolactin (PRL) and its receptor: actions, signal transduction pathways and phenotypes observed in PRL receptor knockout mice. Endocr Rev. 19(3): 225-68.
  • Goffin V, et al. (1999) From the molecular biology of prolactin and its receptor to the lessons learned from knockout mice models. Genet Anal. 15(3-5): 189-201.
  • Li Y, et al. (2006) Stabilization of prolactin receptor in breast cancer cells. Oncogene. 25(13): 1896-902.
  • Shao R, et al. (2008) Differences in prolactin receptor (PRLR) in mouse and human fallopian tubes: evidence for multiple regulatory mechanisms controlling PRLR isoform expression in mice. Biol Reprod. 79(4): 748-57.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Prolactin Receptor Antibody Rabbit Polyclonal ELISA 80062-T16
    Anti-Prolactin Receptor Antibody Rabbit Monoclonal ELISA 80062-R001
    Anti-Prolactin Receptor Antibody Rabbit Monoclonal ELISA 80062-R113
    Anti-Prolactin Receptor Antibody Rabbit Polyclonal ELISA 80062-RP01
    Anti-Prolactin Receptor Antibody Rabbit Polyclonal ELISA 50457-T16
    Anti-Prolactin Receptor Antibody Rabbit Polyclonal ELISA 10278-RP01
    Anti-Prolactin Receptor Antibody (PE) Rabbit Monoclonal FCM 10278-R204-P
    Anti-Prolactin Receptor Antibody Rabbit Monoclonal ELISA(Cap) 10278-R313
    Anti-Prolactin Receptor Antibody (APC) Rabbit Monoclonal FCM 10278-R204-A
    Anti-Prolactin Receptor Antibody Rabbit Polyclonal ELISA,IHC-P 10278-T24
    Anti-Prolactin Receptor Antibody Mouse Monoclonal ELISA 10278-MM13
    Anti-Prolactin Receptor Antibody Rabbit Polyclonal ELISA 10278-RP02
    Anti-Prolactin Receptor Antibody Rabbit Polyclonal IHC-P 10278-T08
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