Mouse TTR Gene cDNA Clone / ORF Clone

TTR cDNA Clone Datasheet

TTR cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013697.5
• cDNA Size: 444
• Gene Synonym: D17860; AA408768; AI787086; MGC107649; prealbumin; Ttr
• Source: Mouse

TTR cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus transthyretin DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

TTR cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

TTR cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

TTR cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTTCCC TTCGACTCTT CCTCCTTTGC CTCGCTGGAC TGGTATTTGT GTCTGAAGCT
61       GGCCCCGCGG GTGCTGGAGA ATCCAAATGT CCTCTGATGG TCAAAGTCCT GGATGCTGTC
121      CGAGGCAGCC CTGCTGTAGA CGTGGCTGTA AAAGTGTTCA AAAAGACCTC TGAGGGATCC
181      TGGGAGCCCT TTGCCTCTGG GAAGACCGCG GAGTCTGGAG AGCTGCACGG GCTCACCACA
241      GATGAGAAGT TTGTAGAAGG AGTGTACAGA GTAGAACTGG ACACCAAATC GTACTGGAAG
301      ACACTTGGCA TTTCCCCGTT CCATGAATTC GCGGATGTGG TTTTCACAGC CAACGACTCT
361      GGCCATCGCC ACTACACCAT CGCAGCCCTG CTCAGCCCAT ACTCCTACAG CACCACGGCT
421      GTCGTCAGCA ACCCCCAGAA TTGA

 

Quality Control:cDNA sequencing results is available upon request.