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Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N ORF mammalian expression plasmid, C-HA tag

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 PRRSV-N cDNA Clone Product Information
NCBI RefSeq:U87392.3
RefSeq ORF Size:372bp
cDNA Description:Full length Clone DNA of Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N with C terminal HA tag.
Gene Synonym:PRRSV-N
Species:PRRSV
Vector:pCMV3-C-HA
Plasmid:
Restriction Site:
Tag Sequence:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N ORF mammalian expression plasmid, C-HA tag on other vectors
Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N ORF mammalian expression plasmid, C-GFPSpark tagVG40337-ACG$325
Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N ORF mammalian expression plasmid, C-OFPSpark / RFP tagVG40337-ACR$325
Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N ORF mammalian expression plasmid, N-GFPSpark tagVG40337-ANG$325
Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N ORF mammalian expression plasmid, N-OFPSpark / RFP tagVG40337-ANR$325
Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedVG40337-CF$295
Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N ORF mammalian expression plasmid, C-His tagVG40337-CH$295
Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N ORF mammalian expression plasmid, C-Myc tagVG40337-CM$295
Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N ORF mammalian expression plasmid, C-HA tagVG40337-CY$295
Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N ORF mammalian expression plasmid (Codon Optimized)VG40337-G$95
Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N ORF mammalian expression plasmid, N-Flag tagVG40337-NF$295
Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N ORF mammalian expression plasmid, N-His tagVG40337-NH$295
Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N ORF mammalian expression plasmid, N-Myc tagVG40337-NM$295
Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N ORF mammalian expression plasmid, N-HA tagVG40337-NY$295
Porcine reproductive and respiratory syndrome virus (PRRSV) (type 2, strain ATCC VR2332 (NA)) N natural ORF mammalian expression plasmidVG40337-UT$295
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