Mouse Pleiotrophin/PTN Gene cDNA Clone / ORF Clone

PTN cDNA Clone Datasheet

PTN cDNA Clone Product Information

• Gene Bank Ref.ID: NM_008973.2
• cDNA Size: 507
• Gene Synonym: OSF; HARP; HBBN; HBNF; Osf1; Osf-1; HB-GAM; HBGF-8; Ptn
• Source: Mouse

PTN cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus pleiotrophin DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 165 C/T not causing the amino acid variation.

PTN cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PTN cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PTN cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCGTCCC AGCAATATCA GCAGCAACGT AGAAAATTTG CAGCTGCCTT CCTGGCATTG
61       ATTTTCATCT TGGCAGCTGT GGACACTGCT GAGGCCGGGA AGAAAGAGAA ACCTGAAAAA
121      AAGGTGAAAA AGTCTGACTG TGGAGAATGG CAGTGGAGTG TGTGTGTGCC TACCAGCGGG
181      GACTGTGGAT TGGGCACCCG GGAGGGCACT CGCACTGGCG CCGAGTGCAA ACAGACCATG
241      AAGACTCAGA GATGTAAGAT CCCTTGCAAC TGGAAGAAGC AGTTTGGAGC TGAGTGCAAG
301      TACCAGTTCC AGGCTTGGGG AGAATGTGAC CTCAATACCG CCTTGAAGAC CAGAACTGGC
361      AGCCTGAAGC GAGCTCTGCA CAATGCTGAC TGTCAGAAAA CTGTCACCAT CTCCAAGCCC
421      TGTGGCAAGC TCACCAAGCC CAAGCCTCAA GCGGAGTCAA AGAAGAAGAA AAAGGAAGGC
481      AAGAAACAGG AGAAGATGCT GGATTAA

 

Quality Control:cDNA sequencing results is available upon request.