Peroxiredoxin 1 / PRDX1 Antibody

Peroxiredoxin 1 / PRDX1 Antibody Datasheet

Peroxiredoxin 1 / PRDX1 Antibody Product Information

• Immunogen: Recombinant Human Peroxiredoxin 1 / PRDX1 protein (Catalog#11194-H08E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human Peroxiredoxin 1 / PRDX1 (rh Peroxiredoxin 1 / PRDX1; Catalog#11194-H08E; NP_002565.1; Met 1-Lys 199). Total IgG was purified by Protein A affinity chromatography.

Peroxiredoxin 1 / PRDX1 Antibody Usage Guide

• Specificity: Human Peroxiredoxin 1 / PRDX1
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human PRDX1 in WB.
• Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human PRDX1. The detection limit for Human PRDX1 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Peroxiredoxin 1 / PRDX1 Antibody Background

Peroxiredoxin-1, also known as Thioredoxin peroxidase 2, Natural killer cell-enhancing factor A, PRDX1, and PAGA, is a member of the ahpC/TSA family. Peroxiredoxin-1 is constitutively expressed in most human cells. It is induced to higher levels upon serum stimulation in untransformed and transformed cells. Peroxiredoxins (PRDXs) are a family of antioxidant enzymes that are also known as scavengers of peroxide in mammalian cells. The overexpression of Peroxiredoxin-1, which is one of the peroxiredoxins that is a ubiquitously expressed protein, was related to a poor prognosis in several types of human cancers. Peroxiredoxin-1 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin and may play an important role in eliminating peroxides generated during metabolism. Peroxiredoxin-1 Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. The reduced Peroxiredoxin-1 expression is an important factor in esophageal squamous cancer progression and could serve as a useful prognostic marker.

References

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4. Cao, J. et al., 2009, EMBO J. 28 (10): 1505-17.