Rat PVRL2 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80327-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PVRL2 cDNA Clone Datasheet

PVRL2 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001012064.1
cDNA Size:	1593
Gene Synonym:	MGC94554; Pvrl2
Source:	Rat

PVRL2 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus poliovirus receptor-related 2 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 120T/C，468T/C and 474G/A not causing the amino acid variation.

PVRL2 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PVRL2 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PVRL2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCCGGG CCGCAGTCCT CCCGCCGTCC AGATTGTCAC CGACGCTGCC GTTGTTGCCG
61       CTGCTACTGC TCCTGCTTCA GGAAACAGGA GCCCAAGATG TGCGGGTACG AGTGCTCCCC
121      GAGGTTCGTG GCCGCCTGGG AGGCACGGTG GAGTTACCAT GCCACCTGCT CCCACCCACG
181      ACGGAGCGCG TCTCTCAGGT GACCTGGCAG CGCCTGGATG GCACAGTTGT GGCTGCCTTC
241      CACCCATCCT TCGGCGTGGA TTTCCCCAAC TCTCAGTTCA ACAAGGACCG TCTGTCCTTC
301      GTCCGAGCGA AACAAGAAGC AAACGCGGAC CTGCGGGACG CCACGCTGGC CTTCCGGGGT
361      CTGAGGGTAG AGGACGAGGG CAATTACACT TGCGAGTTTG CCACGTTTCC CAACGGTACC
421      CGCAGGGGAG TGACCTGGCT CAGAGTCATA GCCCAGCCGG AGAACCACGC TGAAGCCCAA
481      GAGGTCACAA TTGGCCCCCA GTCGGTAGCT GTTGCCCGCT GTATCTCCAC GGGGGGCCGC
541      CCCCCTGCCC GAATCACCTG GATCTCATCC CTGGGTGGAG AGGCCAGAGA TATTCAGGAG
601      CCCGGACTAC AGGCTGGCAC GGTCACTGTC ATCAGTCGAT ACTCCATGGT GCCGGTGGGC
661      CGAGCGGATG GCGTCAAGGT CACGTGCAGA GTGGAACATG AGAGCTTGGA AGAGCCCGAG
721      CTGCTGCCCG TGATCCTCTC CGTGCGCTAC CCTCCCGAAG TATCCATCTC CGGCTATGAT
781      GACAACTGGT ACCTAGGCCG CAGCGAGGCC ACACTGACCT GTGAAGTACG AAGCAACCCA
841      GAGCCTACAG GCTATGACTG GAGCACGACC TCGGGGGTCT TCCCAGCCTC CGCAGTGGCC
901      CAGGGCTCTC AGCTGCTTGT CCACTCTGTG GATCGAATGG TCAACACTAC CTTCATCTGC
961      ACAGCCACCA ATGCTGTGGG GACAGGCCGT GCTGAGCAGG TCATCCTGGT GCGAGAGTCA
1021     CCTAGCACGG CAGGAGCAGG GGCCACGGGA GGCATCATTG GAGGCATCAT CGCTGCCATC
1081     ATCGCCACCG CAGTGGCTGG CACAGGCATC CTCATCTGCC GACAACAGCG GAAGGAGCAG
1141     AGGCTTCAGG CGGCCGACGA GGAAGAAGAA CTGGAAGGAC CTCCCTCCTA TAAGCCACCC
1201     ACCCCAAAGG CCAAGCTGGA GGAACCAGAA ATGCCCTCAC AACTCTTCAC TTTGGGACCC
1261     TCGGAACACA GCCCAGTGAA GACGCCATAC TTTGATGCTG GTGTCTCTTG TGCTGATCAG
1321     GAGATGCCTC GATATCACGA GCTGCCCACC CTGGAAGAGC GGTCAGGGCC CCTGCTGTTA
1381     GGGGCTACAG GCCTGGGACC TTCTCTTCTT GTGCCTCCAG GACCCAATGT TGTGGAGGGG
1441     GTTTCCCTGG GTCTCGAAGA TGAGGAGGAA GATGAGGAGG AGGAAGATTT CCTGGATAAA
1501     ATCAACCCTA TTTATGATGC CCTATCCTAC CCCAGCCCCT CTGACTCCTA CCAGAGCAAA
1561     GACTTTTTTG TGTCACGGGC CATGTATGTG TGA

Quality Control:cDNA sequencing results is available upon request.