Anti-PVRL1 Antibody

Cat: 80244-RP01
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Anti-PVRL1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-PVRL1 Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Rat
Specificity
Rat PVRL1
Immunogen
Recombinant Rat PVRL1 / HVEC / Nectin-1 protein (Catalog#80244-R08H)
Preparation
Produced in rabbits immunized with purified, recombinant Rat PVRL1 / HVEC / Nectin-1 (rR PVRL1 / HVEC / Nectin-1; Catalog#80244-R08H; Met1-Ala354). Total IgG was purified by Protein A affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-PVRL1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

PVRL1 Background Information

Poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin-1; CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV). CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.

Full Name
poliovirus receptor-related 1 (herpesvirus entry mediator C)
References
  • Yoon M, et al. (2002) Disruption of adherens junctions liberates nectin-1 to serve as receptor for herpes simplex virus and pseudorabies virus entry. J Virol. 76(14): 7203-8.
  • Mata M, et al. (2001) HveC (nectin-1) is expressed at high levels in sensory neurons, but not in motor neurons, of the rat peripheral nervous system. J Neurovirol. 7(5): 476-80.
  • Haarr L, et al. (2001) Transcription from the gene encoding the herpesvirus entry receptor nectin-1 (HveC) in nervous tissue of adult mouse. Virology. 287(2): 301-9.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-PVRL1 Antibody Rabbit Polyclonal ELISA 80244-T16
    Anti-PVRL1 Antibody Rabbit Monoclonal ELISA(Cap) 80244-R029
    Anti-PVRL1 Antibody Rabbit Monoclonal ELISA,ELISA(Det) 80244-R004
    Anti-PVRL1 Antibody Rabbit Polyclonal WB,ELISA 11611-RP01
    Anti-PVRL1 Antibody Rabbit Polyclonal ELISA 11611-RP02
    Anti-PVRL1 Antibody (APC) Mouse Monoclonal FCM 11611-MM09-A
    Anti-PVRL1 Antibody Mouse Monoclonal ELISA,ELISA(Det) 11611-MM01
    Anti-PVRL1 Antibody Mouse Monoclonal FCM 11611-MM09
    Anti-PVRL1 Antibody (PE) Mouse Monoclonal FCM 11611-MM09-P
    Anti-PVRL1 Antibody Mouse Monoclonal ELISA(Cap) 11611-MM08
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