Cynomolgus PVR / CD155 Antibody

PVR / CD155 Antibody Datasheet

PVR / CD155 Antibody Product Information

• Immunogen: Recombinant Cynomolgus CD155 protein (Catalog#90005-C08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 4G5D10D1
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Cynomolgus PVR / CD155 (rC PVR / CD155; Catalog#90005-C08H; NP_001036851.1; Met 1-Asn 343). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

PVR / CD155 Antibody Usage Guide

• Specificity: Cynomolgus PVR / CD155
• No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Cynomolgus PVR in WB. Using a DAB detection system, the detection limit for Cynomolgus PVR is approximately 8 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Cynomolgus PVR. The detection limit for Cynomolgus PVR is approximately 0.31 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

PVR / CD155 Antibody Background

CD155, also known as PVR (poliovirus receptor) and Necl-5 (nectin-like molecule-5), is a type I transmembrane single-span glycoprotein, and belongs to the nectins and nectin-like (Necl) subfamily of immunoglobulin superfamily. This protein contains three Ig-like extracellular domains, a transmembrane segment, and a cytoplasmic tail. In humans, CD155 is predominately expressed by enterocytes and cells of gastrointestinal lymphatic tissues and originally identified based on its ability to mediate the cell attachment and entry of poliovirus (PV), an etiologic agent of the central nervous system disease poliomyelitis. The normal cellular function of CD155 maybe the involvement of intercellular adhension between epithelial cells. Alternate splicing of the CD155 mRNA yields four different isoforms (α, β, γ, and δ) with the identical extracellular domains. CD155-β and-γ are secreted, whereas the membrane-bound CD155-α and -δ serve as PV receptors. In addition, it has been demonstrated that CD155 can be recognized and bond by DNAM-1 and CD96 which promote the adhension, migration and NK-cell killing, and thus efficiently prime cell-mediated tumor-specific immunity.

References

1. Mendelsohn, C.L. et al., 1989, Cell. 56: 855-865.
2. He,Y. et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97: 79-84.
3. Simon, K. et al., 2001, J. Virol. 75: 4984-4989.
4. Minami,Y., et al., 2007, J. Biol. Chem. 282: 18481-18496.
5. Fuchs, A. et al., 2004, J. Immunol. 172: 3994-3998.
6. Tahara-Hanaoka,S. et al., 2006, Blood. 107: 1491-1496.