PTX3 / TNFAIP5 Protein (His Tag) (Cytokine)

Pentraxin-Related Protein PTX3 Protein

PTX3 / TNFAIP5 / TSG-14 Protein Product Information

• Synonym: PTX3, TNFAIP5, TSG-14
• Protein Construction: A DNA sequence encoding the human PTX3 ( NP_002843.2 ) ( Met 1 - Ser 381 ) was fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

PTX3 / TNFAIP5 / TSG-14 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 18
• Molecular Mass: The secreted recombinant human PTX3 consists of 375 amino acids and has a predicted molecular mass of 41.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 43 - 47 kDa.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PTX3 protein

PTX3 / TNFAIP5 / TSG-14 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PTX3 / TNFAIP5 / TSG-14 Protein Description

Pentraxin-related protein PTX3, also known as Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein, TSG-14, PTX3 and TNFAIP5, is a secreted protein which contains one pentaxin domain. PTX3 plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. Pentraxins are a family of evolutionarily conserved multifunctional pattern-recognition proteins characterized by a cyclic multimeric structure. Based on the primary structure of the subunit, the pentraxins are divided into two groups: short pentraxins and long pentraxins. C-reactive protein (CRP) and serum amyloid P-component (SAP) are the two short pentraxins. The prototype protein of the long pentraxin group is pentraxin 3 (PTX3). CRP and SAP are produced primarily in the liver in response to IL-6, while PTX3 is produced by a variety of tissues and cells and in particular by innate immunity cells in response to proinflammatory signals and Toll-like receptor (TLR) engagement. PTX3 is essential in female fertility by acting as a nodal point for the assembly of the cumulus oophorus hyaluronan-rich extracellular matrix. PTX3 interacts with several ligands, including growth factors, extracellular matrix components and selected pathogens, playing a role in complement activation and facilitating pathogen recognition by phagocytes, acting as a predecessor of antibodies. PTX3 may also contribute to the pathogenesis of atherosclerosis.

References

1. Rolph,M.S. et al., 2002, Arterioscler Thromb Vasc Biol. 22 (5):e10-4.
2. Mantovani A., et al., 2003, Vaccine. 21:S43-S47.
3. Luchetti,M.M. et al., 2004, Clin Exp Rheumatol. 22 (3):S66-72.
4. Mantovani, A. et al., 2006, Vascul Pharmacol. 45 (5):326-30.
5. Inforzato A., et al., 2008, J. Biol. Chem. 283:10147-61.