PTX3 / Pentraxin 3 / TSG-14 Antibody (Antigen Affinity Purified) (Cytokine)

PTX3 / Pentraxin 3 / TSG-14 Antibody ( Antigen Affinity Purified ) Datasheet

PTX3 / Pentraxin 3 / TSG-14 Antibody Product Information

• Immunogen: Recombinant Human PTX3 / Pentraxin 3 / TSG-14 protein (Catalog#12082-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human PTX3 / Pentraxin 3 / TSG-14 (rh PTX3 / Pentraxin 3 / TSG-14; Catalog#12082-H08H; NP_002843.2; Met 1-Ser 381). PTX3 / Pentraxin 3 / TSG-14 specific IgG was purified by Human PTX3 / Pentraxin 3 / TSG-14 affinity chromatography.

PTX3 / Pentraxin 3 / TSG-14 Antibody Usage Guide

• Specificity: Human PTX3 / Pentraxin 3 / TSG-14
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse PTX3 in WB. Using a DAB detection system, the detection limit for Mouse PTX3 is approximately 0.5 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse PTX3. The detection limit for Mouse PTX3 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

PTX3 / Pentraxin 3 / TSG-14 Antibody Background

Pentraxin-related protein PTX3, also known as Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein, TSG-14, PTX3 and TNFAIP5, is a secreted protein which contains onepentaxin domain. PTX3 plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. Pentraxins are a family of evolutionarily conserved multifunctional pattern-recognition proteins characterized by a cyclic multimeric structure. Based on the primary structure of the subunit, the pentraxins are divided into two groups: short pentraxins and long pentraxins. C-reactive protein (CRP) and serum amyloid P-component (SAP) are the two short pentraxins. The prototype protein of the long pentraxin group is pentraxin 3 (PTX3). CRP and SAP are produced primarily in the liver in response to IL-6, while PTX3 is produced by a variety of tissues and cells and in particular by innate immunity cells in response to proinflammatory signals and Toll-like receptor (TLR) engagement. PTX3 is essential in female fertility by acting as a nodal point for the assembly of the cumulus oophorus hyaluronan-rich extracellular matrix. PTX3 interacts with several ligands, including growth factors, extracellular matrix components and selected pathogens, playing a role in complement activation and facilitating pathogen recognition by phagocytes, acting as a predecessor of antibodies. PTX3 may also contribute to the pathogenesis of atherosclerosis.

References

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