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Human PTH2 / PTRH2 Gene ORF cDNA clone in cloning vector

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Human PTRH2 cDNA Clone Product Information
NCBI RefSeq:BC006807
RefSeq ORF Size:540bp
cDNA Description:Full length Clone DNA of Homo sapiens peptidyl-tRNA hydrolase 2.
Gene Synonym:BIT1, PTH2, CGI-147, PTRH2
Species:Human
Vector:pGEM-T Vector
Plasmid:pGEM-PTRH2
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:
Promoter:
Application:
Antibiotic in E.coli:
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
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Background

PTH2, also known as PTRH2, is a mitochondrial protein which belongs to the PTH2 family. PTH 2 is released during apoptosis from the mitochondria to the cytoplasm. The natural substrate for PTH 2 may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. When in the cytoplasm, PTRH2 regulates the function of 2 transcriptional regulators, TLE5 and TLE1, thus promoting caspase-independent cell death.

References
  • Prahalad AK. et al., 2006, Proteomics. 6 (12): 3482-93.
  • Jin L. et al., 2000, J Biol Chem. 275 (35): 27238-44.
  • Boone T. et al., 2000, Nat Struct Biol. 7 (6): 486-91.
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