|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Homo sapiens peptidyl-tRNA hydrolase 2 DNA.|
|BIT1, PTH2, CGI-147, PTRH2|
|Identical with the Gene Bank Ref. ID sequence.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human PTRH2 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG13645-G-F|
|Human PTRH2 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG13645-G-H|
|Human PTRH2 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG13645-G-M|
|Human PTRH2 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG13645-G-N|
|Human PTRH2 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG13645-G-Y|
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PTH2, also known as PTRH2, is a mitochondrial protein which belongs to the PTH2 family. PTH 2 is released during apoptosis from the mitochondria to the cytoplasm. The natural substrate for PTH 2 may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. When in the cytoplasm, PTRH2 regulates the function of 2 transcriptional regulators, TLE5 and TLE1, thus promoting caspase-independent cell death.