Human PTPRJ Gene cDNA Clone / ORF Clone

PTPRJ cDNA Clone Datasheet

PTPRJ cDNA Clone Product Information

• Gene Bank Ref.ID: NM_002843.3
• cDNA Size: 4014
• Gene Synonym: DEP1, SCC1, CD148, HPTPeta, R-PTP-ETA, PTPRJ
• Source: Human

PTPRJ cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens protein tyrosine phosphatase, receptor type, J DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 699 T/C, 2616 G/C not causing the amino acid variation.

PTPRJ cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PTPRJ cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PTPRJ cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGCCGG CGGCGCGGGA GGCGCGGCTG CCTCCGCGCT CGCCCGGGCT GCGCTGGGCG
61       CTGCCGCTGC TGCTGCTGCT GCTGCGCCTG GGCCAGATCC TGTGCGCAGG TGGCACCCCT
121      AGTCCAATTC CTGACCCTTC AGTAGCAACT GTTGCCACAG GGGAAAATGG CATAACGCAG
181      ATCAGCAGTA CAGCAGAATC CTTTCATAAA CAGAATGGAA CTGGAACACC TCAGGTGGAA
241      ACAAACACCA GTGAGGATGG TGAAAGCTCT GGAGCCAACG ATAGTTTAAG AACACCTGAA
301      CAAGGATCTA ATGGGACTGA TGGGGCATCT CAAAAAACTC CCAGTAGCAC TGGGCCCAGT
361      CCTGTGTTTG ACATTAAAGC TGTTTCCATC AGTCCAACCA ATGTGATCTT AACTTGGAAA
421      AGTAATGACA CAGCTGCTTC TGAGTACAAG TATGTAGTAA AGCATAAGAT GGAAAATGAG
481      AAGACAATTA CTGTTGTGCA TCAACCATGG TGTAACATCA CAGGCTTACG TCCAGCGACT
541      TCATATGTAT TCTCCATCAC TCCAGGAATA GGCAATGAGA CTTGGGGAGA TCCCAGAGTC
601      ATAAAAGTCA TCACAGAGCC GATCCCAGTT TCTGATCTCC GTGTTGCCCT CACGGGTGTG
661      AGGAAGGCTG CTCTCTCCTG GAGCAATGGC AATGGCACCG CCTCCTGCCG GGTTCTTCTT
721      GAAAGCATTG GAAGCCATGA GGAGTTGACT CAAGACTCAA GACTTCAGGT CAATATCTCG
781      GGCCTGAAGC CAGGGGTTCA ATACAACATC AACCCGTATC TTCTACAATC AAATAAGACA
841      AAGGGAGACC CCTTGGGCAC AGAAGGTGGC TTGGATGCCA GCAATACAGA GAGAAGCCGG
901      GCAGGGAGCC CCACCGCCCC TGTGCATGAT GAGTCCCTCG TGGGACCTGT GGACCCATCC
961      TCCGGCCAGC AGTCCCGAGA CACGGAAGTC CTGCTTGTCG GGTTAGAGCC TGGCACCCGA
1021     TACAATGCCA CCGTTTATTC CCAAGCAGCG AATGGCACAG AAGGACAGCC CCAGGCCATA
1081     GAGTTCAGGA CAAATGCTAT TCAGGTTTTT GACGTCACCG CTGTGAACAT CAGTGCCACA
1141     AGCCTGACCC TGATCTGGAA AGTCAGCGAT AACGAGTCGT CATCTAACTA TACCTACAAG
1201     ATACATGTGG CGGGGGAGAC AGATTCTTCC AATCTCAACG TCAGTGAGCC TCGCGCTGTC
1261     ATCCCCGGAC TCCGCTCCAG CACCTTCTAC AACATCACAG TGTGTCCTGT CCTAGGTGAC
1321     ATCGAGGGCA CGCCGGGCTT CCTCCAAGTG CACACCCCCC CTGTTCCAGT TTCTGACTTC
1381     CGAGTGACAG TGGTCAGCAC GACGGAGATC GGCTTAGCAT GGAGCAGCCA TGATGCAGAA
1441     TCATTTCAGA TGCATATCAC ACAGGAGGGA GCTGGCAATT CTCGGGTAGA AATAACCACC
1501     AACCAAAGTA TTATCATTGG TGGCTTGTTC CCTGGAACCA AGTATTGCTT TGAAATAGTT
1561     CCAAAAGGAC CAAATGGGAC TGAAGGGGCA TCTCGGACAG TTTGCAATAG AACTGTTCCC
1621     AGTGCAGTGT TTGACATCCA CGTGGTCTAC GTCACCACCA CGGAGATGTG GCTGGACTGG
1681     AAGAGCCCTG ACGGTGCTTC CGAGTATGTC TACCATTTAG TCATAGAGTC CAAGCATGGC
1741     TCTAACCACA CAAGCACGTA TGACAAAGCG ATTACTCTCC AGGGCCTGAT TCCGGGCACC
1801     TTATATAACA TCACCATCTC TCCAGAAGTG GACCACGTCT GGGGGGACCC CAACTCCACT
1861     GCACAGTACA CACGGCCCAG CAATGTGTCC AACATTGATG TAAGTACCAA CACCACAGCA
1921     GCAACTTTAA GTTGGCAGAA CTTTGATGAC GCCTCTCCCA CGTACTCCTA CTGCCTTCTT
1981     ATTGAGAAGG CTGGAAATTC CAGCAACGCA ACACAAGTAG TCACGGACAT TGGAATTACT
2041     GACGCTACAG TCACTGAATT AATACCTGGC TCATCATACA CAGTGGAGAT CTTTGCACAA
2101     GTAGGGGATG GGATCAAGTC ACTGGAACCT GGCCGGAAGT CATTCTGTAC AGATCCTGCG
2161     TCCATGGCCT CCTTCGACTG CGAAGTGGTC CCCAAAGAGC CAGCCCTGGT TCTCAAATGG
2221     ACCTGCCCTC CTGGCGCCAA TGCAGGCTTT GAGCTGGAGG TCAGCAGTGG AGCCTGGAAC
2281     AATGCGACCC ACCTGGAGAG CTGCTCCTCT GAGAATGGCA CTGAGTATAG AACGGAAGTC
2341     ACGTATTTGA ATTTTTCTAC CTCGTACAAC ATCAGCATCA CCACTGTGTC CTGTGGAAAG
2401     ATGGCAGCCC CCACCCGGAA CACCTGCACT ACTGGCATCA CAGATCCCCC TCCTCCAGAT
2461     GGATCCCCTA ATATTACATC TGTCAGTCAC AATTCAGTAA AGGTCAAGTT CAGTGGATTT
2521     GAAGCCAGCC ACGGACCCAT CAAAGCCTAT GCTGTCATTC TCACCACCGG GGAAGCTGGT
2581     CACCCTTCTG CAGATGTCCT GAAATACACG TATGACGATT TCAAAAAGGG AGCCTCAGAT
2641     ACTTATGTGA CATACCTCAT AAGAACAGAA GAAAAGGGAC GTTCTCAGAG CTTGTCTGAA
2701     GTTTTGAAAT ATGAAATTGA CGTTGGGAAT GAGTCAACCA CACTTGGTTA TTACAATGGG
2761     AAGCTGGAAC CTCTGGGCTC CTACCGGGCT TGTGTGGCTG GCTTCACCAA CATTACCTTC
2821     CACCCTCAAA ACAAGGGGCT CATTGATGGG GCTGAGAGCT ATGTGTCCTT CAGTCGCTAC
2881     TCAGATGCTG TTTCCTTGCC CCAGGATCCA GGTGTCATCT GTGGAGCGGT TTTTGGCTGT
2941     ATCTTTGGTG CCCTGGTTAT TGTGACTGTG GGAGGCTTCA TCTTCTGGAG AAAGAAGAGG
3001     AAAGATGCAA AGAATAATGA AGTGTCCTTT TCTCAAATTA AACCTAAAAA ATCTAAGTTA
3061     ATCAGAGTGG AGAATTTTGA GGCCTACTTC AAGAAGCAGC AAGCTGACTC CAACTGTGGG
3121     TTCGCAGAGG AATACGAAGA TCTGAAGCTT GTTGGAATTA GTCAACCTAA ATATGCAGCA
3181     GAACTGGCTG AGAATAGAGG AAAGAATCGC TATAATAATG TTCTGCCCTA TGATATTTCC
3241     CGTGTCAAAC TTTCGGTCCA GACCCATTCA ACGGATGACT ACATCAATGC CAACTACATG
3301     CCTGGCTACC ACTCCAAGAA AGATTTTATT GCCACACAAG GACCTTTACC GAACACTTTG
3361     AAAGATTTTT GGCGTATGGT TTGGGAGAAA AATGTATATG CCATCATTAT GTTGACTAAA
3421     TGTGTTGAAC AGGGAAGAAC CAAATGTGAG GAGTATTGGC CCTCCAAGCA GGCTCAGGAC
3481     TATGGAGACA TAACTGTGGC AATGACATCA GAAATTGTTC TTCCGGAATG GACCATCAGA
3541     GATTTCACAG TGAAAAATAT CCAGACAAGT GAGAGTCACC CTCTGAGACA GTTCCATTTC
3601     ACCTCCTGGC CAGACCACGG TGTTCCCGAC ACCACTGACC TGCTCATCAA CTTCCGGTAC
3661     CTCGTTCGTG ACTACATGAA GCAGAGTCCT CCCGAATCGC CGATTCTGGT GCATTGCAGT
3721     GCTGGGGTCG GAAGGACGGG CACTTTCATT GCCATTGATC GTCTCATCTA CCAGATAGAG
3781     AATGAGAACA CCGTGGATGT GTATGGGATT GTGTATGACC TTCGAATGCA TAGGCCTTTA
3841     ATGGTGCAGA CAGAGGACCA GTATGTTTTC CTCAATCAGT GTGTTTTGGA TATTGTCAGA
3901     TCCCAGAAAG ACTCAAAAGT AGATCTTATC TACCAGAACA CAACTGCAAT GACAATCTAT
3961     GAAAACCTTG CGCCCGTGAC CACATTTGGA AAGACCAATG GTTACATCGC CTAA

 

Quality Control:cDNA sequencing results is available upon request.