Rat PTPRC transcript variant 1 Gene cDNA Clone / ORF Clone

PTPRC cDNA Clone Datasheet

PTPRC cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001109890.1
• cDNA Size: 3426
• Gene Synonym: Lca; RT7; CD45; L-CA; T200; Ptprc
• Source: Rat

PTPRC cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus protein tyrosine phosphatase, receptor type, C DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 2919 T/A resulting in the amino acid Asp substitution by Glu and 756 A/C, 2922 A/G not causing the amino acid variation.

PTPRC cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PTPRC cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PTPRC cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTATTTGT GGCTCAAACT TCTGGCCTTC AGCTTGGCCC TTCTCGGCCC AGAAGTCTTT
61       GTCACAGGGC AAGGAACAAC CGACGATGGT GCCAACCTCA CAACCCCTGC ACCATCCACT
121      CTGGGCTTTG CAAGCAATAC CACCACAAGC ACAGAAATAG CTACCCCTCA AACGAAGCCA
181      TCATGTGATG AAAAATTTGG GAACGTTACT GTGCGTTACA TCTATGATGA CAGTAGTAAG
241      AATTTTAACG CAAACCTAGA AGGTGACAAA AAACCTAAGT GTGAATATAC GGATTGTGAA
301      AAAGAGTTAA AAAATCTACC AGAATGCTCA CAGAAAAACG TCACTCTCTC CAATGGCTCA
361      TGTACTCCAG ATAAAATTAT AAATTTAGAT GTACCACCAG GGACTCACAA CTTTAACTTA
421      ACAAACTGCA CACCAGACAT AGAAGCTAAT ACCTCAATTT GTTTGGAGTG GAAAATAAAA
481      AACAAATTTA CCTGCGACAT TCAAAAGATT TCATACAATT TCCGTTGTAC ACCAGAGATG
541      AAAACATTTG CTTTGGACAA ACACGGAACA CTTTGGTTAC ACAACCTTAC AGTCCGAACA
601      AATTACACAT GTGCTGCGGA AGTCCTCTAC AATAACGTAA TACTTTTAAA ACAAGACAGA
661      AGGGTGCAGA CTGATTTTGG GACTCCAGAA ATGCTTCCCC ATGTTCAATG TAAGAATTCA
721      ACTAACAGCA CAACATTAGT CTCCTGGGCT GAGCCCGCAT CTAAACACCA TGGATACATT
781      TTATGCTATA AAAAGACCCC TTCAGAAAAA TGTGAAAATT TGGCTAATGA TGTGAACAGT
841      TTTGAGGTGA AAAACCTGAG GCCTTATACA GAGTACACAG TGTCTCTATT TGCCTATGTT
901      ATTGGGAGGG TGCAACGAAA TGGCCCTGCT AAGGATTGCA ACTTTCGGAC AAAAGCAGCT
961      CGTCCAGGCA AAGTCAATGG TATGAAAACC TCCCGGGCGT CAGACAATAG TATAAATGTC
1021     ACGTGTAATT CTCCTTATGA AATTAATGGC CCTGAAGCGC GTTACATTTT GGAAGTCAAA
1081     AGTGGAGGTT CTTTAGTTAA AACTTTCAAC CAATCCACAT GTAAATTTGT TGTAGACAAT
1141     CTCTATTATT CAACTGACTA TGAGTTTCTG GTCTATTTTT ACAATGGAGA GTACCTGGGA
1201     GACCCAGAAA TAAAACCTCA ATCAACATCT TATAATTCTA AAGCACTGAT TATATTCCTG
1261     GTGTTTCTGA TTATTGTGAC ATCAATAGCC CTGCTTGTTG TTTTGTATAA AATCTATGAT
1321     CTGCGTAAGA AAAGATCCAG CAATTTAGAT GAACAGCAGG AACTCGTTGA AAGGGATGAG
1381     GAGAAGCAGC TGATAAATGT GGACCCAATT CATTCTGACC TTTTGTTGGA AACATACAAA
1441     AGAAAGATTG CCGATGAGGG TAGACTGTTC CTGGCTGAAT TTCAGAGCAT TCCACGGGTA
1501     TTCAGCAAGT TTCCCATCAA AGATGCCCGA AAGTCCCAAA ACCAGAACAA AAACCGTTAT
1561     GTGGACATTC TCCCCTATGA TTACAACCGT GTGGAGCTCT CTGAAATAAA TGGAGACGCA
1621     GGGTCCACCT ACATAAATGC CAGCTACATT GATGGCTTCA AGGAACCCAG GAAATACATT
1681     GCTGCACAAG GGCCCCGGGA TGAGACAGTT GATGACTTCT GGAAGATGAT CTGGGAGCAG
1741     AAGGCCACAG TTATTGTCAT GGTCACACGA TGTGAAGAAG GAAACAGGAA CAAGTGTGCA
1801     GAATATTGGC CATGCATGGA GGAAGGCACT CGGACTTTCA GAGATGTTGT CGTGACGATC
1861     AATGACCACA AACGATGTCC TGATTACATT ATCCAGAAGC TGAGCATTGC CCACAAAAAA
1921     GAAAAAGCAA CTGGAAGAGA AGTGACTCAT ATTCAATTCA CCAGTTGGCC AGACCATGGG
1981     GTTCCTGAAG ACCCTCACCT GCTCCTGAAA CTTCGACGGA GAGTTAATGC TTTTAGCAAC
2041     TTCTTCAGTG GACCCATTGT GGTGCACTGC AGTGCTGGCG TTGGGCGTAC AGGCACCTAC
2101     ATTGGAATTG ATGCCATGCT CGAAAGCCTA GAAGCAGAGG GCAAAGTGGA TGTCTATGGC
2161     TATGTTGTCA ACCTAAGGCG ACAGAGATGT CTGATGGTGC AAGTGGAGGC CCAGTACATC
2221     CTGATCCATC AGGCCTTAGT GGAGTACAAT CAATTTGGGG AAACAGAAGT GAACTTGTCT
2281     GAGTTACATT CATGTCTACA GAATCTGAAG AAGAGAGATC CACCCAGTGA CCCGTCTCCT
2341     TTGGAGGCTG AGTACCAGAG ACTTCCTTCA TACAGGAGCT GGAGGACACA GCACATTGGA
2401     AATCAAGAAG AAAATAAAAA GAAAAACAGG AGTTCTAACG TTGTTCCATA TGACTTTAAC
2461     AGAGTGCCAC TTAAGCATGA ACTAGAGATG AGCAAAGAGA GCGAGGCTGA ATCCGACGAA
2521     TCTTCAGATG AGGACAGTGA CTCGGAAGAA ACCAGCAAAT ACATTAATGC GTCATTTGTG
2581     ATGAGTTACT GGAAACCAGA AATGATGATT GCTGCCCAGG GTCCACTAAA AGAGACTATT
2641     GGTGACTTTT GGCAGATGAT ATTCCAAAGA AAAGTCAAGG TTATTGTGAT GTTGACAGAG
2701     TTAATGAGTG GAGACCAGGA AGTCTGTGCT CAATACTGGG GAGAAGGAAA GCAGACTTAT
2761     GGAGACATGG AAGTAATGTT GAAAGACACG AACAAATCCT CAGCCTATAT TCTGCGAGCA
2821     TTTGAACTGA GACATTCCAA GAGAAAGGAG CCTAGAACTG TGTACCAGTA CCAGTGTACC
2881     ACATGGAAAG GGGAGGAGCT CCCTGCAGAA CCCAAAGAAC TGGTGACTCT GATTCAGAAC
2941     ATCAAACAGA AGCTTCCCAA GAGTGGCTCA GAAGGGATGA AGTACCACAA GCATGCATCA
3001     ATCCTAGTCC ACTGCAGGGA TGGATCCCAG CAGACGGGGT TGTTCTGTGC TCTGTTCAAT
3061     CTCCTGGAAA GTGCAGAAAC AGAAGATGTG GTTGATGTTT TTCAAGTGGT AAAGTCTCTA
3121     CGCAAAGCAC GGCCGGGGAT GGTGGGCAGC TTTGAGCAAT ACCAGTTCCT CTATGACATC
3181     ATGGCCAGCA TCTATCCCAC CCAGAATGGA CAAGTGAAGA AAGCAAACAG CCAAGACAAA
3241     ATTGAATTTC ATAACGAAGT GGACGGAGCC AAGCAGGACG CAAACTGTGT TCAGCCAGCT
3301     GATCCTCTGA ACAAAGCCCA GGAAGACAGC AAAGAAGTTG GAGCTTCAGA GCCTGCAAGT
3361     GGTTCTGAGG AGCCAGAACA TTCTGCAAAT GGTCCCATGA GCCCAGCTCT AACCCCGAGC
3421     TCATAG

 

Quality Control:cDNA sequencing results is available upon request.