PTPRA / PTPalpha (aa 174-793) Protein (GST Tag)

Protein tyrosine phosphatase, receptor type, A Protein Datasheet

PTPRA / PTPalpha (aa 174-793) Protein Product Information

• Synonym: RP4-534B8.1, HEPTP, HLPR, HPTPA, HPTPalpha, LRP, PTPA, PTPRL2, R-PTP-alpha, RPTPA
• Protein Construction: A DNA sequence encoding the human PTPRA isoform 2 (?P18433-2) cytoplasmic domain (Ala 174-Lys 793) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

PTPRA / PTPalpha (aa 174-793) Protein QC Testing

• Purity: >95 % as determined by SDS-PAGE.
• Bio-activity: 1. Measured by its ability to bind biotinylated recombinant mouse SRC in a functional ELISA.
  2. Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is >40000 pmol/min/μg.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human PTPRA(aa 174-793) /GST chimera consists of 857 amino acids and has a calculated molecular mass of 99 KDa. It migrates as an approximately 90 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 20%gly, 3mM DTT
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PTPRA / PTPalpha (aa 174-793) protein

PTPRA / PTPalpha (aa 174-793) Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PTPRA / PTPalpha (aa 174-793) Protein Description

Protein tyrosine phosphatase, receptor type, A (PTPRA) also named PTPalpha, is a transmembrane member of the extensive family of protein-tyrosine phosphatases (PTPs). PTPRA/PTPalpha is constitutively phosphorylated on Tyr789, a consensus binding site for the SH2 domain of the SH3-SH2-SH3 adaptor protein GRB2. Human PTPRA/PTPalpha consists of 802 amino acids. The extracellular domain of 150 residues includes a hydrophobic signal peptide and eight potential N-glycosylation sites. This is followed by a transmembrane region and two tandemly repeated conserved domains. PTPRA/PTPalpha mediates NB-3 that plays an important role in the apical dendrite development of layer V pyramidal neurons in the visual cortex. Cortical neurons from PTPRA/PTPalpha knock-out mice exhibited a lower level of NB-3 at the cell surface. Co-expression of PTPRA/PTPalpha increased the cell surface distribution of NB-3. Further analysis showed that PTPRA/PTPalpha facilitated Golgi exit of NB-3 and stabilized NB-3 protein at the cell surface by preventing its release from the plasma membrane. The extracellular region of PTPRA/PTPalpha but not its catalytic activity is necessary for its effect on NB-3 expression.

References

1. R Kaplan. et al., 1990, Proc Natl Acad Sci. 87 (18): 7000-4.
2. Hertog JD. et al., 1996, EMBO J. 15 (12): 3016-27.
3. Ye H. et al., 2011, J Biol Chem. 286 (29): 26071-80.