Mouse PTPN6 Gene cDNA Clone / ORF Clone

PTPN6 cDNA Clone Datasheet

PTPN6 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001077705.1
• cDNA Size: 1794
• Gene Synonym: me; hcp; Hcph; Ptp1C; SHP-1; motheaten; Ptpn6
• Source: Mouse

PTPN6 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus protein tyrosine phosphatase, non-receptor type 6 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PTPN6 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PTPN6 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PTPN6 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTGTCCC GCGGGTGGTT TCACCGGGAC CTCAGCGGGC CTGATGCAGA GACCCTGCTG
61       AAGGGCCGGG GAGTCCCTGG GAGCTTCCTG GCTCGGCCCA GCCGCAAGAA CCAGGGTGAC
121      TTCTCCCTCT CAGTCAGGGT GGATGATCAG GTGACTCATA TTCGGATCCA GAACTCAGGG
181      GACTTCTATG ACCTGTACGG AGGGGAGAAG TTTGCGACGC TGACAGAGCT GGTCGAGTAT
241      TACACGCAGC AGCAGGGCAT CCTGCAGGAC CGAGATGGCA CCATCATCCA CCTTAAGTAC
301      CCACTGAACT GCTCGGACCC CACCAGTGAG AGGTGGTACC ACGGCCACAT ATCTGGAGGG
361      CAGGCGGAGT CACTGCTGCA GGCCAAGGGC GAGCCCTGGA CATTTCTTGT GCGTGAGAGT
421      CTCAGCCAAC CTGGTGATTT TGTGCTCTCT GTGCTCAATG ACCAGCCCAA GGCTGGCCCA
481      GGTTCCCCGC TCAGGGTCAC TCATATCAAG GTTATGTGTG AGGGTGGACG CTATACTGTG
541      GGTGGCTCAG AGACGTTTGA CAGCCTCACA GACCTGGTGG AGCACTTCAA GAAGACAGGG
601      ATTGAGGAGG CCTCGGGTGC CTTTGTCTAC CTGCGGCAGC CTTACTACGC TACTCGGGTA
661      AACGCAGCTG ACATTGAGAA TCGGGTCTTG GAACTGAACA AGAAGCAGGA GTCGGAGGAC
721      ACAGCCAAGG CTGGCTTCTG GGAGGAGTTT GAGAGTCTAC AAAAGCAGGA GGTAAAGAAT
781      CTACACCAAC GTCTGGAAGG GCAGCGGCCA GAGAACAAGA GCAAGAACCG CTACAAGAAC
841      ATTCTTCCCT TTGACCACAG CCGAGTGATC CTGCAGGGAC GTGACAGTAA CATCCCAGGC
901      TCTGACTACA TCAATGCCAA CTACGTGAAG AACCAGCTGC TAGGTCCAGA TGAGAACTCT
961      AAGACCTACA TCGCCAGCCA GGGCTGTCTG GATGCCACAG TCAATGACTT CTGGCAGATG
1021     GCTTGGCAGG AGAACACTCG TGTCATCGTC ATGACTACCA GAGAGGTGGA GAAAGGCCGG
1081     AACAAATGTG TCCCATACTG GCCCGAGGTG GGCACTCAGC GTGTCTATGG TCTCTACTCT
1141     GTGACCAACA GTAGGGAGCA TGACACAGCA GAATACAAAC TGCGAACATT ACAGATCTCC
1201     CCACTAGACA ATGGGGACCT GGTTCGGGAG ATATGGCACT ACCAGTACCT GAGCTGGCCT
1261     GACCATGGGG TTCCCAGTGA GCCTGGGGGT GTCCTCAGCT TTCTGGATCA GATCAACCAG
1321     CGACAGGAAA GTTTGCCTCA TGCAGGGCCC ATCATTGTGC ATTGCAGCGC TGGCATCGGC
1381     CGCACGGGCA CCATCATCGT CATTGATATG CTTATGGAAA GCATCTCCAC CAAGGGGCTA
1441     GACTGTGACA TTGATATCCA GAAGACCATC CAGATGGTAC GAGCACAGCG CTCCGGCATG
1501     GTGCAGACCG AGGCCCAGTA CAAGTTTATT TACGTGGCCA TTGCCCAGTT CATCGAAACG
1561     ACCAAGAAGA AACTGGAGAT CATACAATCC CAGAAGGGCC AGGAGTCGGA GTATGGGAAT
1621     ATCACGTACC CTCCCGCTGT GAGGAGTGCC CACGCCAAAG CCTCGCGTAC TTCCTCCAAG
1681     CACAAGGAGG AGGTGTACGA AAACGTGCAT AGCAAGAGCA AGAAGGAAGA GAAAGTAAAG
1741     AAGCAGCGGT CGGCAGACAA GGAGAAGAAC AAAGGTTCTC TCAAGAGGAA GTGA

 

Quality Control:cDNA sequencing results is available upon request.