|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Homo sapiens protein tyrosine phosphatase, non-receptor type 11 DNA.|
|CFC, NS1, SHP2, BPTP3, PTP2C, PTP-1D, SH-PTP2, SH-PTP3, PTPN11|
|Identical with the Gene Bank Ref. ID sequence.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human PTPN11 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG12318-G-F|
|Human PTPN11 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG12318-G-H|
|Human PTPN11 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG12318-G-M|
|Human PTPN11 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG12318-G-N|
|Human PTPN11 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG12318-G-Y|
|Product name||Product name|
SHP2, also known as PTPN11, belongs to the protein-tyrosine phosphatase(PTP) family, non-receptor class 2 subfamily. PTPs catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They dephosphorylate EGFR, JAK2 and TYK2 kinases, promoting oncogenic transformation. SHP2 is widely expressed, with highest levels in heart, brain, and skeletal muscle. SHP2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. It also dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity.