Human PTPN11 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12318-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PTPN11 cDNA Clone Datasheet

PTPN11 cDNA Clone Product Information

Gene Bank Ref.ID:	BC008692
cDNA Size:	1383
Gene Synonym:	CFC; NS1; SHP2; BPTP3; PTP2C; PTP-1D; SH-PTP2; SH-PTP3；PTPN11
Source:	Human

PTPN11 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens protein tyrosine phosphatase, non-receptor type 11 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PTPN11 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PTPN11 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PTPN11 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACATCGC GGAGATGGTT TCACCCAAAT ATCACTGGTG TGGAGGCAGA AAACCTACTG
61       TTGACAAGAG GAGTTGATGG CAGTTTTTTG GCAAGGCCTA GTAAAAGTAA CCCTGGAGAC
121      TTCACACTTT CCGTTAGAAG AAATGGAGCT GTCACCCACA TCAAGATTCA GAACACTGGT
181      GATTACTATG ACCTGTATGG AGGGGAGAAA TTTGCCACTT TGGCTGAGTT GGTCCAGTAT
241      TACATGGAAC ATCACGGGCA ATTAAAAGAG AAGAATGGAG ATGTCATTGA GCTTAAATAT
301      CCTCTGAACT GTGCAGATCC TACCTCTGAA AGGTGGTTTC ATGGACATCT CTCTGGGAAA
361      GAAGCAGAGA AATTATTAAC TGAAAAAGGA AAACATGGTA GTTTTCTTGT ACGAGAGAGC
421      CAGAGCCACC CTGGAGATTT TGTTCTTTCT GTGCGCACTG GTGATGACAA AGGGGAGAGC
481      AATGACGGCA AGTCTAAAGT GACCCATGTT ATGATTCGCT GTCAGGAACT GAAATACGAC
541      GTTGGTGGAG GAGAACGGTT TGATTCTTTG ACAGATCTTG TGGAACATTA TAAGAAGAAT
601      CCTATGGTGG AAACATTGGG TACAGTACTA CAACTCAAGC AGCCCCTTAA CACGACTCGT
661      ATAAATGCTG CTGAAATAGA AAGCAGAGTT CGAGAACTAA GCAAATTAGC TGAGACCACA
721      GATAAAGTCA AACAAGGCTT TTGGGAAGAA TTTGAGACAC TACAACAACA GGAGTGCAAA
781      CTTCTCTACA GCCGAAAAGA GGGTCAAAGG CAAGAAAACA AAAACAAAAA TAGATATAAA
841      AACATCCTGC CCTTTGATCA TACCAGGGTT GTCCTACACG ATGGTGATCC CAATGAGCCT
901      GTTTCAGATT ACATCAATGC AAATATCATC ATGCCTGAAT TTGAAACCAA GTGCAACAAT
961      TCAAAGCCCA AAAAGAGTTA CATTGCCACA CAAGGCTGCC TGCAAAACAC GGTGAATGAC
1021     TTTTGGCGGA TGGTGTTCCA AGAAAACTCC CGAGTGATTG TCATGACAAC GAAAGAAGTG
1081     GAGAGAGGAA AGAGTAAATG TGTCAAATAC TGGCCTGATG AGTATGCTCT AAAAGAATAT
1141     GGCGTCATGC GTGTTAGGAA CGTCAAAGAA AGCGCCGCTC ATGACTATAC GCTAAGAGAA
1201     CTTAAACTTT CAAAGGTTGG ACAAGGGAAT ACGGAGAGAA CGGTCTGGCA ATACCACTTT
1261     CGGACCTGGC CGGACCACGG CGTGCCCAGC GACCCTGGGG GCGTGCTGGA CTTCCTGGAG
1321     GAGGTGCACC ATAAGCAGGA GAGCATCATG GATGCAGGGC CGGTCGTGGT GCACTGCAGG
1381     TGA

Quality Control:cDNA sequencing results is available upon request.