PTPMT1 Protein (His Tag)

Protein tyrosine phosphatase, mitochondrial 1 Protein Datasheet

PTPMT1 Protein Product Information

• Synonym: PNAS-129, DUSP23, FLJ46081, MOSP, PLIP
• Protein Construction: A DNA sequence encoding the human PTPMT1 isoform 1 (Q8WUK0-1) (Lys 28-Thr 201) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

PTPMT1 Protein QC Testing

• Purity: > 94 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human PTPMT1 consisting of 190 amino acids and has a calculated molecular mass of 21.7 KDa. It migrates as an 20 kDa band in SDS-PAGE under reducing conditions as predicted.
• Formulation: Lyophilized from 0.2μm filtered solution of PBS , 10%glycerol, 1mM DTT pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PTPMT1 protein

PTPMT1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PTPMT1 Protein Description

PTPMT1 (PTP localized to the Mitochondrion 1) is a member of the protein tyrosine phosphatase superfamily that is localized exclusively to the mitochondrion. It has been recently reported that PTPMT1 dephosphorylates phosphatidylglycerol phosphate, an essential intermediate of cardiolipin biosynthesis. PTPMT1 deficiency in mouse embryonic fibroblasts compromises mitochondrial respiration and results in abnormal mitochondrial morphology. Lipid analysis of PTPMT1-deficient fibroblasts reveals an accumulation of PGP along with a concomitant decrease in phosphatidylglycerol. Modulation of mitochondrial ATP synthesis by PTPMT1 suggests a novel approach for the treatment of pancreatic cancers, which represent some of the deadliest forms of human tumors. The gluttony of cancer cells for energy is well established, and with the development of a modulator of expression, one may hope that we could also achieve the synthetic induction of PTPMT1 expression. It would then be expected that this effect would attenuate, if not abolish, the growth of pancreas-derived tumor cells and support the establishment of a novel regimen for pancreatic cancers.

References

1. Zhang J. et al., 2011, Cell Metab. 13 (6): 690-700.
2. Boisclair Y. et al., 2005, Mol Cell. 19 (3): 291-2.
3. Xiao J. et al., 2011, Proc Natl Acad Sci. 108 (29): 11860-5.