PTP1B / PTPN1 Antibody

PTP1B / PTPN1 Antibody Datasheet

PTP1B / PTPN1 Antibody Product Information

• Immunogen: Recombinant Human PTP1B / PTPN1 protein (Catalog#10304-H07E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human PTP1B / PTPN1 (rh PTP1B / PTPN1; Catalog#10304-H07E; NP_002818.1; Glu 2-Asn 321). Total IgG was purified by Protein A affinity chromatography.

PTP1B / PTPN1 Antibody Usage Guide

• Specificity: Human PTP1B / PTPN1
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human PTPN1. The detection limit for Human PTPN1 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

PTP1B / PTPN1 Antibody Background

Protein tyrosine phosphatase 1B (PTP1B) is the prototypic member of the PTP family which catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues, and is identified based on the enzymatic activity and amino acid sequence. PTP1B/PTPN1 is a ubiquitously expressed non-transmembrane enzyme which resides in the endoplasmic reticulum with its conserved catalytic regions exposed to the cytosol. PTP1B/PTPN1 is reported to dephosphorylates a variety of substrates including receptor tyrosine kinases (RTKs) on the cell surface transmitting critical intracellular signals, as well as JAK2, Tyk2 and STAT5a/b, and thus plays a key role in negatively regulating the multiple tyrosine phosphorylation-dependent signaling pathways. Accordingly, this enzyme is implicated in the regulation of cell growth, differentiation, cell responses to cytokines and hormones, and malignancy transformation. In addition, it has been demonstrated that the CLK kinases activate PTP1B/PTPN1 can be activated by the CLK kinases. PTP1B/PTPN1 is now suggested to be a novel therapeutic target for the treatment of type 2 diabetes, obesity, and other associated metabolic syndromes.

References

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