Anti-PTP1B Antibody (Rabbit Monoclonal antibody) General Information
Reacts with: Human
Recombinant Human PTP1B / PTPN1 protein (Catalog#10304-H07E)
This antibody was obtained from a rabbit immunized with purified, recombinant Human PTP1B / PTPN1 (rh PTP1B / PTPN1; Catalog#10304-H07E; NP_002818.1; Glu 2-Asn 321).
Monoclonal Rabbit IgG Clone #001
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-PTP1B Antibody (Rabbit Monoclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-PTP1B Antibody (Rabbit Monoclonal antibody) Images
Immunofluorescence staining of human PTPN1 in A549 and Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-human PTPN1 monoclonal antibody (1:60) at 4℃ overnight. Then A549 cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (figure A, captured by fluorescence microscope); Hela cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (figure B, captured by laser confocal scanning microscope) , countstained with DAPI (blue). Positive staining was localized to cytoplasm.
Anti-PTP1B Antibody Alternative Names
Anti-PTP1B Antibody;Anti-PTPN1 Antibody
PTP1B Background Information
PTP1B, also known as PTPN1, belongs to the protein-tyrosine phosphatase (PTP) family. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTP1B contains 1 tyrosine-protein phosphatase domain and is expressed in many tissues. PTP1B is localized to the cytoplasmic face of the endoplasmic reticulum. PTP1B was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of PTP1B in cell growth control, and cell response to IFN stimulation.
protein tyrosine phosphatase, non-receptor type 1
Frangioni JV, et al. (1992) The nontransmembrane tyrosine phosphatase PTP-1B localizes to the endoplasmic reticulum via its 35 amino acid C-terminal sequence. Cell. 68(3):545-60.Zhu S, et al. (2007) PTP1B contributes to the oncogenic properties of colon cancer cells through Src activation. Cancer Res. 67(21):10129-37.Aoki N, et al. (2000) A cytosolic protein-tyrosine phosphatase PTP1B specifically dephosphorylates and deactivates prolactin-activated STAT5a and STAT5b. J Biol Chem. 275(50):39718-26.Stuible M, et al. (2008) PTP1B regulates cortactin tyrosine phosphorylation by targeting Tyr446. J Biol Chem. 283(23):15740-6.