Anti-PTP1B Antibody

Cat: 10304-RP02
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Anti-PTP1B Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-PTP1B Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human PTP1B
Immunogen
Recombinant Human PTP1B / PTPN1 protein (Catalog#10304-H07E)
Preparation
Produced in rabbits immunized with purified, recombinant Human PTP1B / PTPN1 (rh PTP1B / PTPN1; Catalog#10304-H07E; NP_002818.1; Glu 2-Asn 321). PTP1B / PTPN1 specific IgG was purified by Human PTP1B / PTPN1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-PTP1B Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IHC-P 1:2000-1:10000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-PTP1B Antibody (Rabbit Polyclonal antibody) Images
Human PTP1B / PTPN1 Immunohistochemistry(IHC) 3716
Immunochemical staining of human PTPN1 in human tonsil with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Human PTP1B / PTPN1 Immunohistochemistry(IHC) 3717
Immunochemical staining of human PTPN1 in human breast cancer with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Human PTP1B / PTPN1 Immunoprecipitation(IP) 7970

PTPN1 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

0.5 µL anti-PTPN1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-PTPN1 rabbit polyclonal antibody,at 1:350 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 50 kDa

Observed band size: 50 kDa

Human PTP1B / PTPN1 Western blot (WB) 13176

Anti-PTPN1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: HepG2 Whole Cell Lysate

Lane C: A431 Whole Cell Lysate

Lane D: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:50 kDa

Anti-PTP1B Antibody Alternative Names
Anti-PTP1B Antibody;Anti-PTPN1 Antibody
PTP1B Background Information

PTP1B, also known as PTPN1, belongs to the protein-tyrosine phosphatase (PTP) family. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTP1B contains 1 tyrosine-protein phosphatase domain and is expressed in many tissues. PTP1B is localized to the cytoplasmic face of the endoplasmic reticulum. PTP1B was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of PTP1B in cell growth control, and cell response to IFN stimulation.

Full Name
protein tyrosine phosphatase, non-receptor type 1
References
  • Frangioni JV, et al. (1992) The nontransmembrane tyrosine phosphatase PTP-1B localizes to the endoplasmic reticulum via its 35 amino acid C-terminal sequence. Cell. 68(3):545-60.
  • Zhu S, et al. (2007) PTP1B contributes to the oncogenic properties of colon cancer cells through Src activation. Cancer Res. 67(21):10129-37.
  • Aoki N, et al. (2000) A cytosolic protein-tyrosine phosphatase PTP1B specifically dephosphorylates and deactivates prolactin-activated STAT5a and STAT5b. J Biol Chem. 275(50):39718-26.
  • Stuible M, et al. (2008) PTP1B regulates cortactin tyrosine phosphorylation by targeting Tyr446. J Biol Chem. 283(23):15740-6.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-PTP1B Antibody Rabbit Monoclonal WB,ELISA,IP 10304-R226
    Anti-PTP1B Antibody Rabbit Polyclonal ELISA 10304-RP01
    Anti-PTP1B Antibody Rabbit Monoclonal ELISA,ICC/IF,IF 10304-R001
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