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PTP1B / PTPN1 Antibody, Rabbit PAb, Antigen Affinity Purified
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PTP1B / PTPN1 Antibody, Rabbit PAb, Antigen Affinity Purified PDF Download

Catalog   Size (Price) Quantity In Stock Operation
10304-RP02  

YES   
 

Primary antibody | Secondary antibody | Tag antibody | Isotype control antibody | Loading control antibody | Antibody Purification

PTP1B / PTPN1 Antibody ( Antigen Affinity Purified ) Datasheet

  Order or Inquire for PTP1B / PTPN1 Antibody product Quality antibodies Antibody production services
  Detection limit is 0.039 ng/well in ELISA
 

PTP1B / PTPN1 Antibody Product Information

Immunogen :

Recombinant Human PTP1B / PTPN1 protein (Catalog#10304-H07E)

Antibody Type : Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type :

Rabbit IgG

Formulation : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
Preparation :

Produced in rabbits immunized with purified, recombinant Human PTP1B / PTPN1 (rh PTP1B / PTPN1; Catalog#10304-H07E; NP_002818.1; Glu 2-Asn 321). PTP1B / PTPN1 specific IgG was purified by Human PTP1B / PTPN1 affinity chromatography.

PTP1B / PTPN1 Antibody Usage Guide

Specificity :

Human PTP1B / PTPN1

Immunochemistry :

IHC-P: 0.1-2 μg/mL

 

PTPN1-breast-cancer immunohistochemistry    PTPN1-tonsil immunohistochemistry

Immunochemical staining of human PTPN1 in human breast cancer with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections). Immunochemical staining of human PTPN1 in human tonsil with rabbit polyclonal antibody (1 μg/mL, formalin-fixed paraffin embedded sections).
Western blot :

WB: 10-20 μg/mL

 

PTP1B / PTPN1 Western blot

 

  A B C D E F
Sample
(whole cell lysate)
Hela 293T Jurkat HL-60 TF-1 HepG2
Sample Volume
(μg/lane)
30 35 30
Gel 13% SDS-PAGE reducing gel
Recommended Concentration 10-20 μg/ml
Secondary Antibody Dylight 800-labeled Antibody To Rabbit IgG (H+L), at 1:5000 dilution.
Direct ELISA : This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human PTPN1. The detection limit for Human PTPN1 is less than 0.039 ng/well.
Storage : This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

PTP1B / PTPN1 Antibody Related Products & Topics

Related Areas:

Enzyme>>Phosphatase & Regulator>>PTP1B/PTPN1

Signal Transduction>>Phosphatase & Regulator>>PTP1B/PTPN1

Proteins:

Molecule Species Description //For Detailed Info. and Price------CLICK! Cat. No
PTP1B/PTPN1 Human PTP1B/PTPN1 Protein, Recombinant 10304-H07E

Antibodies:

Molecule Application Description //For Detailed Info. and Price------CLICK! Cat. No
Human PTP1B/PTPN1 ELISA PTP1B / PTPN1 Antibody 10304-R001
Human PTP1B/PTPN1 ELISA PTP1B / PTPN1 Antibody 10304-RP01
Human PTP1B/PTPN1 ELISA, IHC-P PTP1B / PTPN1 Antibody (Antigen Affinity Purified) 10304-RP02
Human PTP1B/PTPN1 WB, ELISA PTP1B / PTPN1 Antibody 10304-R226

PTP1B / PTPN1 Antibody Background

Protein tyrosine phosphatase 1B (PTP1B) is the prototypic member of the PTP family which catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues, and is identified based on the enzymatic activity and amino acid sequence. PTP1B/PTPN1 is a ubiquitously expressed non-transmembrane enzyme which resides in the endoplasmic reticulum with its conserved catalytic regions exposed to the cytosol. PTP1B/PTPN1 is reported to dephosphorylates a variety of substrates including receptor tyrosine kinases (RTKs) on the cell surface transmitting critical intracellular signals, as well as JAK2, Tyk2 and STAT5a/b, and thus plays a key role in negatively regulating the multiple tyrosine phosphorylation-dependent signaling pathways. Accordingly, this enzyme is implicated in the regulation of cell growth, differentiation, cell responses to cytokines and hormones, and malignancy transformation. In addition, it has been demonstrated that the CLK kinases activate PTP1B/PTPN1 can be activated by the CLK kinases. PTP1B/PTPN1 is now suggested to be a novel therapeutic target for the treatment of type 2 diabetes, obesity, and other associated metabolic syndromes.

References

  1. Charbonneau, H. et al., 1989, Proc. Natl. Acad. Sci. 86: 5252-5256.
  2. Franqioni, J.V. et al., 1992, Cell. 68: 545-560.
  3. Moeslein, F.M. et al., 1999, J. Biol. Chem. 274: 26697-26704.
  4. Haj, F.G. et al., 2002, Science. 295: 1708-1711.
  5. Liang, F. et al., 2005, J. Biol. Chem. 280: 24857-24863.
  6. Zhu, S. et al., 2007, Cancer. Res. 67: 10129-10137.
 

 

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