|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Homo sapiens parathyroid hormone 1 receptor DNA.|
|PFE, PTHR, PTHR1, PTH1R|
|Identical with the Gene Bank Ref. ID sequence.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human PTH1R Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG12232-G-F|
|Human PTH1R Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG12232-G-H|
|Human PTH1R Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG12232-G-M|
|Human PTH1R Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG12232-G-N|
|Human PTH1R Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG12232-G-Y|
|Product name||Product name|
Parathyroid hormone / parathyroid hormone-related peptide receptor, also known as PTH / PTHrP type I receptor, PTH/PTHr receptor, Parathyroid hormone 1 receptor, PTH1 receptor, PTH1R and PTHR, is a multi-pass membrane protein which belongs to the G-protein coupled receptor 2 family. PTH1R is expressed in most tissues. It is most abundant in kidney, bone and liver. PTH1R is expressed in high levels in bone and kidney and regulates calcium ion homeostasis through activation of adenylate cyclase and phospholipase C. In bone, PTH1R is expressed on the surface of osteoblasts. When the receptor is activated, these cells in turn stimulate osteoclasts to ultimately increase the resorption rate. PTH1R is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of PTH1R is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in PTH1R are the cause of Jansen metaphyseal chondrodysplasia (JMC), chondrodysplasia Blomstrand type (BOCD), enchondromatosis multiple (ENCHOM), Eiken skeletal dysplasia (EISD) and primary failure of tooth eruption (PFE).