PTH1R / PTHR1 Antibody (Antigen Affinity Purified)

PTH1R / PTHR1 Antibody ( Antigen Affinity Purified ) Datasheet

PTH1R / PTHR1 Antibody Product Information

• Immunogen: Recombinant Human PTH1R / PTHR1 protein (Catalog#12232-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose

PTH1R / PTHR1 Antibody Usage Guide

• Specificity: Human PTH1R / PTHR1
• Immunochemistry: IHC-P: 0.5-2 μg/mL

  

  Immunochemical staining of human PTH1R in human kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to nephric tubule. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

  

  Immunochemical staining of human PTH1R in human mammary adenocarcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

  Immunochemical staining of human PTH1R in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human PTH1R in WB. Using a DAB detection system, the detection limit for Human PTH1R is approximately 2 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human PTH1R. The detection limit for Human PTH1R is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

PTH1R / PTHR1 Antibody Background

Parathyroid hormone / parathyroid hormone-related peptide receptor, also known as PTH / PTHrP type I receptor, PTH/PTHr receptor, Parathyroid hormone 1 receptor, PTH1 receptor, PTH1R and PTHR, is a multi-pass membrane protein which belongs to theG-protein coupled receptor 2 family. PTH1R is expressed in most tissues. It is most abundant in kidney, bone and liver. PTH1R is expressed in high levels in bone and kidney and regulates calcium ion homeostasis through activation of adenylate cyclase and phospholipase C. In bone, PTH1R is expressed on the surface of osteoblasts. When the receptor is activated, these cells in turn stimulate osteoclasts to ultimately increase the resorption rate. PTH1R is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of PTH1R is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in PTH1R are the cause of Jansen metaphyseal chondrodysplasia (JMC), chondrodysplasia Blomstrand type (BOCD), enchondromatosis multiple (ENCHOM), Eiken skeletal dysplasia (EISD) and primary failure of tooth eruption (PFE).

References

1. Schipani E., et al., 1993, Endocrinology 132: 2157-2165.
2. Hopyan S., et al., 2002, Nat. Genet. 30: 306-310.
3. Bastepe M., et al., 2004, J. Clin. Endocrinol. Metab. 89: 3595-3600.
4. Duchatelet S., et al., 2005, Hum. Mol. Genet. 14: 1-5.
5. Decker E., et al., 2008, Am. J. Hum. Genet. 83: 781-786.