Human PTGDS Gene cDNA Clone / ORF Clone

PTGDS cDNA Clone Datasheet

PTGDS cDNA Clone Product Information

• Gene Bank Ref.ID: BC005939
• cDNA Size: 573
• Gene Synonym: PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS; PTGDS
• Source: Human

PTGDS cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens prostaglandin D2 synthase 21kDa (brain) DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PTGDS cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PTGDS cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PTGDS cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTACTC ATCACACGCT GTGGATGGGA CTGGCCCTGC TGGGGGTGCT GGGCGACCTG
61       CAGGCAGCAC CGGAGGCCCA GGTCTCCGTG CAGCCCAACT TCCAGCAGGA CAAGTTCCTG
121      GGGCGCTGGT TCAGCGCGGG CCTCGCCTCC AACTCGAGCT GGCTCCGGGA GAAGAAGGCG
181      GCGTTGTCCA TGTGCAAGTC TGTGGTGGCC CCTGCCACGG ATGGTGGCCT CAACCTGACC
241      TCCACCTTCC TCAGGAAAAA CCAGTGTGAG ACCCGAACCA TGCTGCTGCA GCCCGCGGGG
301      TCCCTCGGCT CCTACAGCTA CCGGAGTCCC CACTGGGGCA GCACCTACTC CGTGTCAGTG
361      GTGGAGACCG ACTACGACCA GTACGCGCTG CTGTACAGCC AGGGCAGCAA GGGCCCTGGC
421      GAGGACTTCC GCATGGCCAC CCTCTACAGC CGAACCCAGA CCCCCAGGGC TGAGTTAAAG
481      GAGAAATTCA CCGCCTTCTG CAAGGCCCAG GGCTTCACAG AGGATACCAT TGTCTTCCTG
541      CCCCAAACCG ATAAGTGCAT GACGGAACAA TAG

 

Quality Control:cDNA sequencing results is available upon request.