PTGDS / L-PGDS Protein (His Tag)

Prostaglandin D2 synthase 21kDa (brain) Protein Datasheet

PTGDS / L-PGDS Protein Product Information

• Synonym: PTGDS, PDS
• Protein Construction: A DNA sequence encoding the human PTGDS (P41222) (Met1-Gln190) was expressed with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

PTGDS / L-PGDS Protein QC Testing

• Purity: > 80% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 23
• Molecular Mass: The recombinant human PTGDS consists of 179 amino acids and predicts a molecular mass of 20.1 KDa. It migrates as an approximately 28 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PTGDS / L-PGDS protein

PTGDS / L-PGDS Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PTGDS / L-PGDS Protein Description

PTGDS, also known as L-PGDS, is a member of the calycin superfamily, lipocalin family. It is abundantly expressed in the heart, brain, and central nervous system(CNS), where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. PTGDS is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of PGH2 to PGD2. It is involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. It binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter.

References

1. Aebersold R. et al., 1993, Theor Electrophor. 3: 229-34.
2. Oliver K. et al., 2004, Nature. 429: 369-74.
3. Bonaldo MF. et al., 1997, Genome Res. 6 (9): 791-806.