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Human PTEN Gene cDNA Clone (full-length ORF Clone)
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Human PTEN Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG10421-M


  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

Antibody Production Services
Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_000314.4

cDNA Size:   1212

Synonym:  BZS, MHAM, TEP1, MMAC1, PTEN1, 10q23del, MGC11227, PTEN

Source:  Human. cDNA Description:  ORF Clone of Homo sapiens phosphatase and tensin homolog (PTEN) DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG


Sequence Description:  Identical with the Gene Bank Ref. ID sequence. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15–30 min .
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:

1        ATGACAGCCA TCATCAAAGA GATCGTTAGC AGAAACAAAA GGAGATATCA AGAGGATGGA
61 TTCGACTTAG ACTTGACCTA TATTTATCCA AACATTATTG CTATGGGATT TCCTGCAGAA
121 AGACTTGAAG GCGTATACAG GAACAATATT GATGATGTAG TAAGGTTTTT GGATTCAAAG
181 CATAAAAACC ATTACAAGAT ATACAATCTT TGTGCTGAAA GACATTATGA CACCGCCAAA
241 TTTAATTGCA GAGTTGCACA ATATCCTTTT GAAGACCATA ACCCACCACA GCTAGAACTT
301 ATCAAACCCT TTTGTGAAGA TCTTGACCAA TGGCTAAGTG AAGATGACAA TCATGTTGCA
361 GCAATTCACT GTAAAGCTGG AAAGGGACGA ACTGGTGTAA TGATATGTGC ATATTTATTA
421 CATCGGGGCA AATTTTTAAA GGCACAAGAG GCCCTAGATT TCTATGGGGA AGTAAGGACC
481 AGAGACAAAA AGGGAGTAAC TATTCCCAGT CAGAGGCGCT ATGTGTATTA TTATAGCTAC
541 CTGTTAAAGA ATCATCTGGA TTATAGACCA GTGGCACTGT TGTTTCACAA GATGATGTTT
601 GAAACTATTC CAATGTTCAG TGGCGGAACT TGCAATCCTC AGTTTGTGGT CTGCCAGCTA
661 AAGGTGAAGA TATATTCCTC CAATTCAGGA CCCACACGAC GGGAAGACAA GTTCATGTAC
721 TTTGAGTTCC CTCAGCCGTT ACCTGTGTGT GGTGATATCA AAGTAGAGTT CTTCCACAAA
781 CAGAACAAGA TGCTAAAAAA GGACAAAATG TTTCACTTTT GGGTAAATAC ATTCTTCATA
841 CCAGGACCAG AGGAAACCTC AGAAAAAGTA GAAAATGGAA GTCTATGTGA TCAAGAAATC
901 GATAGCATTT GCAGTATAGA GCGTGCAGAT AATGACAAGG AATATCTAGT ACTTACTTTA
961 ACAAAAAATG ATCTTGACAA AGCAAATAAA GACAAAGCCA ACCGATACTT TTCTCCAAAT
1021 TTTAAGGTGA AGCTGTACTT CACAAAAACA GTAGAGGAGC CGTCAAATCC AGAGGCTAGC
1081 AGTTCAACTT CTGTAACACC AGATGTTAGT GACAATGAAC CTGATCATTA TAGATATTCT
1141 GACACCACTG ACTCTGATCC AGAGAATGAA CCTTTTGATG AAGATCAGCA TACACAAATT
1201 ACAAAAGTCT GA

Quality Control:   cDNA sequencing results is available upon request.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"