Human PTCHD1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13211-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PTCHD1 cDNA Clone Datasheet

PTCHD1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_173495.2
cDNA Size:	2667
Gene Synonym:	FLJ30296; MGC149798；PTCHD1
Source:	Human

PTCHD1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens patched domain containing 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 858 T/C not causing the amino acid variation.

PTCHD1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PTCHD1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PTCHD1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGCGGC AGGTTCTGCA CAGGGGCTTG AGGACGTGTT TCTCCCGGCT CGGCCACTTC
61       ATTGCCAGTC ACCCTGTCTT CTTCGCCTCG GCGCCGGTGC TCATCTCCAT CCTGCTCGGC
121      GCCAGCTTCA GCCGCTACCA GGTCGAGGAG AGCGTGGAGC ACCTGCTGGC GCCCCAGCAC
181      AGCCTGGCCA AGATCGAGCG CAACCTCGTT AACAGCCTCT TCCCGGTCAA CCGCTCCAAG
241      CACCGTCTCT ACTCGGACCT GCAGACCCCC GGGCGCTACG GCCGGGTCAT CGTCACCTCC
301      TTCCAGAAAG CCAACATGCT GGACCAGCAT CACACCGACC TGATCTTAAA GTTGCATGCT
361      GCTGTCACCA AGATCCAGGT TCCAAGGCCT GGTTTTAATT ACACGTTTGC CCATATATGT
421      ATCCTGAATA ATGATAAGAC TTGCATCGTG GATGACATAG TGCACGTCCT GGAAGAGCTA
481      AAGAATGCTC GGGCCACCAA TCGGACCAAT TTTGCTATCA CATACCCAAT CACTCACTTA
541      AAGGACGGGA GGGCTGTGTA CAATGGGCAC CAGCTTGGGG GCGTCACTGT GCACAGCAAA
601      GACCGGGTGA AATCTGCAGA GGCCATCCAG CTCACCTACT ACCTGCAGTC AATCAACAGT
661      CTCAATGACA TGGTGGCTGA GAGGTGGGAG TCCAGCTTCT GCGACACTGT CAGACTGTTT
721      CAGAAATCCA ACAGCAAAGT CAAAATGTAC CCTTACACGT CCTCCTCACT GAGGGAAGAT
781      TTCCAGAAGA CCAGCCGCGT ATCAGAACGT TACCTGGTCA CCAGCCTGAT TCTGGTGGTT
841      ACCATGGCCA TCCTGTGCTG CTCTATGCAG GACTGCGTCC GCAGCAAACC CTGGCTAGGC
901      CTGCTCGGAT TGGTGACCAT AAGCCTGGCC ACTCTCACTG CAGCCGGGAT CATCAATCTT
961      ACTGGTGGGA AATATAATTC CACCTTCCTG GGAGTCCCTT TCGTCATGCT AGGTCATGGA
1021     TTATATGGGA CTTTTGAAAT GTTATCCTCC TGGAGGAAAA CTAGAGAAGA CCAACATGTT
1081     AAAGAGAGAA CTGCAGCAGT CTATGCAGAC TCCATGCTCT CCTTTTCTCT CACCACTGCC
1141     ATGTACCTGG TCACCTTTGG CATAGGGGCC AGCCCTTTCA CGAACATTGA GGCAGCCAGG
1201     ATTTTCTGCT GCAATTCCTG TATTGCAATC TTCTTCAACT ACCTCTATGT ACTCTCGTTT
1261     TATGGTTCCA GCCTAGTGTT CACTGGCTAC ATAGAAAACA ATTACCAGCA TAGTATCTTC
1321     TGTAGAAAAG TCCCAAAGCC TGAGGCATTG CAGGAGAAGC CGGCATGGTA CAGGTTTCTC
1381     CTGACGGCCA GATTCAGTGA GGACACAGCT GAAGGCGAGG AAGCGAACAC TTACGAGAGT
1441     CACCTATTGG TATGTTTCCT CAAACGCTAT TACTGTGACT GGATAACCAA CACCTATGTC
1501     AAGCCTTTTG TAGTTCTCTT TTACCTTATT TATATTTCCT TTGCCTTAAT GGGCTATCTG
1561     CAGGTCAGTG AAGGGTCAGA CCTTAGTAAC ATTGTAGCAA CCGCGACACA AACCATTGAG
1621     TACACTACTG CCCAGCAAAA GTACTTCAGC AACTACAGTC CTGTGATTGG GTTTTACATA
1681     TATGAGTCTA TAGAATACTG GAACACTAGT GTCCAAGAAG ATGTTCTAGA ATACACCAAG
1741     GGGTTTGTGC GGATATCCTG GTTTGAGAGC TATTTAAATT ACCTTCGGAA ACTCAATGTA
1801     TCCACTGGCT TGCCTAAGAA AAATTTCACA GACATGTTGA GGAATTCCTT TCTGAAAGCC
1861     CCTCAATTTT CACATTTTCA AGAGGACATC ATCTTCTCTA AAAAATACAA TGATGAGGTC
1921     GATGTAGTGG CCTCCAGAAT GTTTTTGGTG GCCAAGACCA TGGAAACAAA CAGAGAAGAA
1981     CTCTATGATC TCTTGGAAAC CCTGAGGAGA CTTTCTGTCA CCTCCAAGGT GAAGTTCATC
2041     GTCTTCAATC CGTCCTTTGT ATACATGGAT CGATATGCCT CCTCTCTGGG AGCCCCCCTG
2101     CACAACTCCT GCATCAGTGC TTTGTTCCTG CTCTTCTTCT CGGCATTCCT GGTGGCAGAT
2161     TCACTGATTA ACGTCTGGAT CACTCTCACA GTTGTGTCCG TGGAGTTTGG AGTGATAGGT
2221     TTCATGACAT TATGGAAAGT AGAACTGGAC TGCATTTCTG TGCTATGCTT AATTTATGGA
2281     ATTAATTACA CAATTGACAA TTGTGCTCCA ATGTTATCCA CATTTGTTCT GGGCAAGGAT
2341     TTCACAAGAA CTAAATGGGT AAAAAATGCC CTGGAAGTGC ATGGGGTAGC TATTTTACAG
2401     AGTTACCTCT GCTATATTGT TGGTCTGATT CCTCTTGCAG CTGTGCCTTC AAATCTGACC
2461     TGTACACTGT TCAGGTGCTT GTTTTTAATA GCATTTGTCA CCTTCTTTCA CTGCTTTGCC
2521     ATTTTACCTG TGATACTGAC TTTCCTGCCA CCCTCTAAGA AAAAAAGGAA AGAGAAGAAA
2581     AATCCTGAGA ACCGGGAGGA AATTGAGTGT GTAGAAATGG TAGATATCGA TAGTACCCGT
2641     GTGGTTGACC AAATTACAAC AGTGTGA

Quality Control:cDNA sequencing results is available upon request.