Anti-PTBP1 Antibody

Cat: 101043-T46
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Anti-PTBP1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-PTBP1 Antibody
Validated applications
WB,IHC-P,ICC/IF,IF,IP
Species reactivity
Reacts with: Human
Specificity
Human PTBP1
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human PTBP1
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human PTBP1, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-PTBP1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
IHC-P 1:1000-1:5000  
ICC-IF 1:300-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-PTBP1 Antibody (Rabbit Polyclonal antibody) Images
Human PTBP1 Western blot (WB) 14599

Anti-PTBP1 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: Hela Whole Cell Lysate

Lane D: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:57 kDa

Observed band size:57 kDa

Human PTBP1 Immunohistochemistry(IHC) 14600
Immunochemical staining of human PTBP1 in human kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
Human PTBP1 Immunohistochemistry(IHC) 14601
Immunochemical staining of human PTBP1 in human placenta with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
Human PTBP1 Immunohistochemistry(IHC) 14602
Immunochemical staining of human PTBP1 in human breast carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
Human PTBP1 Immunofluorescence(IF) 14603
Immunofluorescence staining of PTBP1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PTBP1 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.
Human PTBP1 Immunoprecipitation(IP) 14604

PTBP1 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg HepG2 Whole Cell Lysate

4 µL anti-PTBP1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-PTBP1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 57 kDa

Observed band size: 57 kDa

Human PTBP1 Immunohistochemistry(IHC) 17999
Immunochemical staining of human PTBP1 in mouse kidney with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.
Human PTBP1 Immunohistochemistry(IHC) 18002
Immunochemical staining of human PTBP1 in mouse small intestine with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.
Human PTBP1 Immunohistochemistry(IHC) 18004
Immunochemical staining of human PTBP1 in rat kidney with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.
Anti-PTBP1 Antibody Alternative Names
Anti-HNRNP-I Antibody;Anti-HNRNPI Antibody;Anti-HNRPI Antibody;Anti-pPTB Antibody;Anti-PTB Antibody;Anti-PTB-1 Antibody;Anti-PTB-T Antibody;Anti-PTB2 Antibody;Anti-PTB3 Antibody;Anti-PTB4 Antibody
PTBP1 Background Information
Full Name
polypyrimidine tract binding protein 1
Product Description Host Clonality Application Catalog# (PDF)
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