Human PSMF1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13480-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PSMF1 cDNA Clone Datasheet

PSMF1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC029836
cDNA Size:	669
Gene Synonym:	PI31; PSMF1
Source:	Human

PSMF1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens proteasome (prosome, macropain) inhibitor subunit DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 667-669 TGA/TGC resulting in the amino acid stop substitution by cys and 474 C/T not causing the amino acid variation.

PSMF1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PSMF1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PSMF1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCTAAGA AGCTTGGGCC CAAATCTCAG AGACAGCGTG GGGCTGTTGC CCCCCTCACC
61       CAGGTCCAGC AGGCGGCCTG TTCCCCCACT TATAAACAGG CCAAGCGCAA TGCCAGGAGC
121      TATCAGGGCC GCCGCCGCCG CCGTCGTTGC AGCCAGAATA CCACTTCCAG GGTTCCTAGC
181      CAACTGCAAG CAGTTGACTC TTCATCTGCT TCCTGCAGGA CCTACAAGAA CAGTGAGGAG
241      CTTCGGTCTC GTATTGTGTC TGGAATCATC ACACCTATCC ATGAGCAGTG GGAAAAGGCT
301      AATGTAAGCA GTCCCCACCG GGAGTTCCCC CCTGCTACCG CCAGAGAGGT GGACCCACTC
361      CGGATTCCTC CACACCACCC ACACACCAGT CGGCAGCCTC CCTGGTGTGA TCCCCTGGGC
421      CCGTTTGTTG TCGGGGGAGA AGACTTAGAC CCTTTTGGGC CTCGGAGAGG TGGTATGATT
481      GTGGATCCCC TGAGATCTGG CTTCCCAAGA GCACTTATTG ACCCTTCCTC AGGCCTCCCG
541      AACCGACTTC CTCCAGGCGC TGTGCCCCCA GGAGCTCGCT TTGACCCCTT TGGACCCATT
601      GGGACCAGCC CACCCGGACC TAACCCAGAC CATCTCCCCC CGCCGGGCTA CGATGACATG
661      TACCTGTGC

Quality Control:cDNA sequencing results is available upon request.